Adenylate Kinase as a Virulence Factor ofPseudomonas aeruginosa
Open Access
- 1 June 2001
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (11), 3345-3352
- https://doi.org/10.1128/jb.183.11.3345-3352.2001
Abstract
Adenylate kinase (AK; ATP:AMP phosphotransferase, EC2.7.4.3) is a ubiquitous enzyme that contributes to the homeostasis of adenine nucleotides in eukaryotic and prokaryotic cells. AK catalyzes the reversible reaction Mg · ATP + AMP ↔ Mg · ADP + ADP. In this study we show that AK secreted by the pathogenic strains ofPseudomonas aeruginosaappears to play an important role in macrophage cell death. We purified and characterized AK from the growth medium of a cystic fibrosis isolate strain ofP. aeruginosa8821 and hyperproduced it as a fusion protein with glutathioneS-transferase. We demonstrated enhanced macrophage cell death in the presence of both the secreted and recombinant purified AK and its substrates AMP plus ATP or ADP. These data suggested that AK converts its substrates to a mixture of AMP, ADP, and ATP, which are potentially more cytotoxic than ATP alone. In addition, we observed increased macrophage killing in the presence of AK and ATP alone. Since the presence of ATPase activity on the macrophages was confirmed in the present work, external macrophage-effluxed ATP is converted to ADP, which in turn can be transformed by AK into a cytotoxic mixture of three adenine nucleotides. Evidence is presented in this study that secreted AK was detected in macrophages during infection withP. aeruginosa.Thus, the possible role of secreted AK as a virulence factor is in producing and keeping an intact pool of toxic mixtures of AMP, ADP, and ATP, which allowsP. aeruginosato exert its full virulence.Keywords
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