Nucleic Acid-Functionalized Metal–Organic Framework-Based Homogeneous Electrochemical Biosensor for Simultaneous Detection of Multiple Tumor Biomarkers
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- 13 February 2019
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 91 (5), 3604-3610
- https://doi.org/10.1021/acs.analchem.8b05599
Abstract
Simultaneous detection of multiple tumor biomarkers is in great demand for early and accurate cancer diagnosis. Homogeneous electrochemical biosensor has been proved to possess high sensitivity, but achieving simultaneous detection of multiple tumor biomarkers is still a challenge. Herein, we develop a novel homogeneous electrochemical biosensor for simultaneous detection of multiple tumor biomarkers based on the functionalized metal-organic frameworks (MOFs). The functionalized MOFs were prepared by using porous UIO-66-NH2 as nanocontainer to load electroactive dyes, and dsDNA as gatekeeper to cap MOFs. In this context, two functionalized MOFs (MB@UIO and TMB@UIO) were fabricated and applied to simultaneous detection of let-7a and miRNA-21, used as the proof-of-concept analytes. The recognition and hybridization of PX with target miRNAs impel the generation of RNA-DNA complexes, which separated from MOFs and allowed the electroactive dyes to be released. In comparison with the case when target miRNAs are absent, two stronger signals are recorded, and dependent on target miRNAs concentrations. Thus, simultaneous detection of let-7a and minRNA-21 is achieved, with detection limits down to 3.6 fM and 8.2 fM, respectively, comparable or lower than those of reported strategies that concentrated on single miRNA detection. Moreover, the proposed biosensor has also been successfully applied for simultaneous detection of target miRNAs spiked in human serum. Therefore, the proposed strategy was expected to provide more information for early and accurate cancer diagnosis, and made useful application in disease diagnosis and clinical biomedicine.Keywords
Funding Information
- National Natural Science Foundation of China (21575074, 21605093, 21775082)
- Shandong Province (ts201511052)
- Natural Science Foundation of Shandong Province (ZR2018ZC0127)
This publication has 37 references indexed in Scilit:
- Graphene Fluorescence Switch-Based Cooperative Amplification: A Sensitive and Accurate Method to Detection MicroRNAAnalytical Chemistry, 2014
- Novel Composite Material Polyoxovanadate@MIL-101(Cr): A Highly Efficient Electrocatalyst for Ascorbic Acid OxidationACS Applied Materials & Interfaces, 2013
- Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal AmplificationJournal of the American Chemical Society, 2013
- Electrocatalytically Active Graphene–Porphyrin MOF Composite for Oxygen Reduction ReactionJournal of the American Chemical Society, 2012
- MOF-Derived Hierarchically Porous Carbon with Exceptional Porosity and Hydrogen Storage CapacityChemistry of Materials, 2012
- Simultaneous Detection of Intracellular Tumor mRNA with Bi‐Color Imaging Based on a Gold Nanoparticle/Molecular BeaconChemistry – A European Journal, 2011
- Phosphorescent Nanoscale Coordination Polymers as Contrast Agents for Optical ImagingAngewandte Chemie-International Edition, 2011
- Zeolitic Imidazolate Framework-8 Nanocrystal Coated Capillary for Molecular Sieving of Branched Alkanes from Linear Alkanes along with High-Resolution Chromatographic Separation of Linear AlkanesJournal of the American Chemical Society, 2010
- Horseradish peroxidase-functionalized Pt hollow nanospheres and multiple redox probes as trace labels for a sensitive simultaneous multianalyte electrochemical immunoassayChemical Communications, 2010