Control of cell adhesion and detachment using temperature and thermoresponsive copolymer grafted culture surfaces
- 22 January 2004
- journal article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 69A (1), 70-78
- https://doi.org/10.1002/jbm.a.20114
Abstract
The hydrophobic monomer, n‐butyl methacrylate (BMA) has been incorporated into thermoresponsive poly(N‐isopropylacrylamide) (PIPAAm) to lower PIPAAm phase transition temperatures necessary for systematically regulating cell adhesion on and detachment from culture dishes at controlled temperatures. Poly(IPAAm‐co‐BMA)‐grafted dishes were prepared by electron beam irradiation methods, systematically changing BMA content in the feed. Copolymer‐grafted surfaces decreased grafted polymer transition temperatures with increasing BMA content as shown by water wettabilities compared to homopolymer PIPAAm‐grafted surfaces. Bovine endothelial cells readily adhered and proliferated on copolymer‐grafted surfaces above collapse temperature at 37°C, finally reaching confluence. Cell sheet detachment behavior from copolymer‐grafted surfaces depended on the culture temperature and BMA content. In conclusion, cell attachment/detachment can be controlled to an arbitrary temperature by varying the content of hydrophobic monomer incorporated into PIPAAm grafted to culture surfaces. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 70–78, 2004Keywords
This publication has 36 references indexed in Scilit:
- Intelligent thermoresponsive polymeric stationary phases for aqueous chromatography of biological compoundsProgress in Polymer Science, 2002
- Temperature-Responsive Liquid Chromatography. 2. Effects of Hydrophobic Groups in N-Isopropylacrylamide Copolymer-Modified SilicaAnalytical Chemistry, 1997
- Thermo-Responsive Polymer Surfaces for Cell Culture: Analysis of the Surfaces and Control of the Cell Attachment / DetachmentPublished by Springer Science and Business Media LLC ,1996
- Temperature-Responsive Chromatography Using Poly(N-isopropylacrylamide)-Modified SilicaAnalytical Chemistry, 1996
- Effect of Molecular Architecture of Poly(N-isopropylacrylamide)−Trypsin Conjugates on Their Solution and Enzymatic PropertiesBioconjugate Chemistry, 1996
- Control of protein–ligand recognition using a stimuli-responsive polymerNature, 1995
- “On–Off” Thermocontrol of Solute Transport. I. Temperature Dependence of Swelling of N-Isopropylacrylamide Networks Modified with Hydrophobic Components in WaterPharmaceutical Research, 1991
- “On–Off“ Thermocontrol of Solute Transport. II. Solute Release from Thermosensitive HydrogelsPharmaceutical Research, 1991
- Temperature dependence of swelling of crosslinked poly(N,N′‐alkyl substituted acrylamides) in waterJournal of Polymer Science Part B: Polymer Physics, 1990
- Solution Properties of Poly(N-isopropylacrylamide)Journal of Macromolecular Science: Part A - Chemistry, 1968