The Enzymatic Synthesis and Anti-HIV-1 Activity of 9-β-D-2′,3′-Dideoxynucleosides of N(6)-Substituted Purines
- 1 August 1990
- journal article
- Published by SAGE Publications in Antiviral Chemistry and Chemotherapy
- Vol. 1 (4), 249-253
- https://doi.org/10.1177/095632029000100404
Abstract
9-β-D-2′,3′-dideoxyribonucleosides of four N(6)-substituted purines and 1-deazapurine were prepared using crude nucleoside N-deoxyribosyltransferases (EC 2.4.2.6) from Lactobacillus leichmannii. The 2′,3′-dideoxynucleosides derived from N(6)-substituted purines (1–4) are inhibitors of the replication of human immunodeficiency virus type 1 (HIV-1), with EC90 values in the range 20–100 and 20–200 μm, depending on the cell line used, and have low in vitro toxicity at their effective concentrations.Keywords
This publication has 13 references indexed in Scilit:
- The Enzymatic Synthesis of Imidazole Deoxynucleosides: 1-β-D-2′-Deoxyribofuranosyl-5-aminoiinidazole-4-carboxamide and 1-β-D-2′-DeoxyribofuranosylbenzimidazoleNucleosides and Nucleotides, 1990
- The stereoselective enzymatic synthesis of 9-β-d-2′-deoxyribofuranosyl 1-deazapurineNucleic Acids Research, 1989
- Comparative activity of 2′,3′-saturated and unsaturated pyrimidine and purine nucleosides against human immunodeficiency virus type 1 in peripheral blood mononuclear cellsBiochemical Pharmacology, 1988
- Synthesis of 2′,3′-dideoxynucleosides by enzymatic trans-glycosylationBiochemical and Biophysical Research Communications, 1988
- Rapid and automated tetrazolium-based colorimetric assay for the detection of anti-HIV compoundsJournal of Virological Methods, 1988
- Principles of Antiretroviral Therapy for AIDS and Related DiseasesPublished by Springer Science and Business Media LLC ,1988
- The Toxicity of Azidothymidine (AZT) in the Treatment of Patients with AIDS and AIDS-Related ComplexNew England Journal of Medicine, 1987
- Broad spectrum antiretroviral activity of 2',3'-dideoxynucleosides.Proceedings of the National Academy of Sciences of the United States of America, 1987
- Antiviral activity of 2′,3′-dideoxycytidin-2′-ene (2′,3′-dideoxy-2′,3′-didehydrocytidine) against human immunodeficiency virusBiochemical Pharmacology, 1987
- 3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro.Proceedings of the National Academy of Sciences of the United States of America, 1985