High-speed, two-photon scanning microscope
- 1 October 1999
- journal article
- Published by Optica Publishing Group in Applied Optics
- Vol. 38 (28), 6004-6009
- https://doi.org/10.1364/ao.38.006004
Abstract
We have developed a high-speed two-photon microscope with submicrometer resolution in real time. The imaging speed improvement of this system is obtained by the use of a high-speed polygonal mirror scanner. The maximum achievable scanning rate is 40 µs/line, which is approximately 100 times faster than conventional scanning microscopes. High-resolution fluorescence images were recorded in real time by an intensified CCD camera. Using this instrument, we have resolved cellular architecture in three dimensions and have monitored the movements of protozoas. More important, photodamage to biological specimens during video-rate imaging can be minimized with two-photon excitation as compared with other one-photon modalities.Keywords
This publication has 9 references indexed in Scilit:
- Real time two‐photon absorption microscopy using multi point excitationJournal of Microscopy, 1998
- Two-photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structuresOptics Express, 1998
- Multifocal multiphoton microscopyOptics Letters, 1998
- Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skinBiophysical Journal, 1997
- Direct Measurement of Coupling Between Dendritic Spines and ShaftsScience, 1996
- Real‐time two‐photon confocal microscopy using a femtosecond, amplified Ti:sapphire systemJournal of Microscopy, 1996
- Quantitative Subcellular Imaging of Glucose Metabolism within Intact Pancreatic IsletsPublished by Elsevier BV ,1996
- In Vivo Confocal Scanning Laser Microscopy of Human Skin: Melanin Provides Strong ContrastJournal of Investigative Dermatology, 1995
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990