Nanobodies and recombinant binders in cell biology
Open Access
- 8 June 2015
- journal article
- review article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 209 (5), 633-644
- https://doi.org/10.1083/jcb.201409074
Abstract
Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, including structural biology, advanced microscopy, and intracellular expression. Understanding these novel reagents as genetic modules that can be integrated into cellular pathways opens up a broad experimental spectrum to monitor and manipulate cellular processes.Keywords
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