Changes in the SDS elutability of fibrinogen adsorbed from plasma to polymers
- 1 January 1989
- journal article
- Published by Taylor & Francis Ltd in Journal of Biomaterials Science, Polymer Edition
- Vol. 1 (2), 99-110
- https://doi.org/10.1163/156856289x00091
Abstract
Baboon fibrinogen was adsorbed from diluted plasma solutions to glass, polyethylene, polystyrene, polydimethylsiloxane, poly(ethylene)terephthalate, and Biomer®. Following adsorption, half of the samples were immediately placed in sodium dodecyl sulfate (SDS) solutions to elute the protein, while the others were stored in buffer for up to 5 days and then eluted in SDS. The elution was typically incomplete, but depended on the plasma concentration and the residence time. The elutability was generally lower for fibrinogen adsorbed from more diluted plasma, and substantially lower for samples on which the fibrinogen had resided for 5 days. The non-elutable portion of the protein layer formed rapidly on polystyrene, while on polyethylene elutability was high initially, followed by a gradual decrease.Keywords
This publication has 17 references indexed in Scilit:
- The kinetics of baboon fibrinogen adsorption to polymers: In vitro and in vivo studiesJournal of Biomedical Materials Research, 1986
- Phenomenology and mechanism of the transient adsorption of fibrinogen from plasma (Vroman effect)Journal of Colloid and Interface Science, 1986
- Sequential protein adsorption and thrombus deposition on polymeric biomaterialsJournal of Colloid and Interface Science, 1986
- The effect of high molecular weight kininogen on surface-adsorbed fibrinogenThrombosis Research, 1984
- Adsorption of proteins from plasma to a series of hydrophilic‐hydrophobic copolymers. II. Compositional analysis with the prelabeled protein techniqueJournal of Biomedical Materials Research, 1981
- Adsorption of fibrinogen on glass: reversibility aspectsJournal of Colloid and Interface Science, 1981
- Adsorption of proteins from plasma to a series of hydrophilic-hydrophobic copolymers. I. Analysis with thein situ radioiodination techniqueJournal of Biomedical Materials Research, 1981
- Dependence of albumin–fibrinogen simple and competitive adsorption on surface properties of biomaterialsJournal of Polymer Science: Polymer Symposia, 1979
- A circular dichroism technique for the study of adsorbed protein structureJournal of Colloid and Interface Science, 1974
- The conformation of adsorbed blood proteins by infrared bound fraction measurementsJournal of Colloid and Interface Science, 1974