Three Siderophores from One Bacterial Enzymatic Assembly Line
- 25 March 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 131 (14), 5056-5057
- https://doi.org/10.1021/ja900815w
Abstract
Siderophores play a vital role in the survival of bacteria, as they facilitate the transport of iron in low-concentration environments. Nature employs a variety of coordinating functional groups in siderophore scaffolds as a way of creating structural diversity. We have successfully shown that the pseudomonine synthetase can produce three distinct siderophore natural products and five siderophore-like compounds. The in vitro enzymatic production of acinetobactin has prompted a revision of the reported structure from an oxazoline to an isoxazolidinone. Our results reveal the inherent flexibility of the pseudomonine synthetase and thus provide insight into the evolution of siderophore biosynthetic gene clusters in bacteria.This publication has 14 references indexed in Scilit:
- Siderophores in Fungal Physiology and VirulenceAnnual Review of Phytopathology, 2008
- A Latent Oxazoline Electrophile for N−O−C Bond Formation in Pseudomonine BiosynthesisJournal of the American Chemical Society, 2008
- How to tailor non-ribosomal peptide products—new clues about the structures and mechanisms of modifying enzymesMolecular BioSystems, 2008
- How pathogenic bacteria evade mammalian sabotage in the battle for ironNature Chemical Biology, 2006
- Enterobactin: An archetype for microbial iron transportProceedings of the National Academy of Sciences of the United States of America, 2003
- Genetics and Assembly Line Enzymology of Siderophore Biosynthesis in BacteriaMicrobiology and Molecular Biology Reviews, 2002
- Analysis of the pmsCEAB Gene Cluster Involved in Biosynthesis of Salicylic Acid and the Siderophore Pseudomonine in the Biocontrol Strain Pseudomonas fluorescens WCS374Journal of Bacteriology, 2001
- Purification of yersiniabactin: a siderophore and possible virulence factor of Yersinia enterocoliticaJournal of General Microbiology, 1993
- Pyochelin: novel structure of an iron-chelating growth promoter for Pseudomonas aeruginosa.Proceedings of the National Academy of Sciences of the United States of America, 1981
- Distribution and renal excretion of desferrioxamine and ferrioxamine in the dog and in the ratBiochemical Pharmacology, 1966