Identification and characterization of a type III secretion-associated chaperone in the type III secretion system 1 ofVibrio parahaemolyticus
Open Access
- 1 July 2009
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 296 (1), 18-25
- https://doi.org/10.1111/j.1574-6968.2009.01607.x
Abstract
Vibrio parahaemolyticus causes human gastroenteritis. Genomic sequencing of this organism has revealed that it has two sets of type III secretion systems, T3SS1 and T3SS2, both of which are important for its pathogenicity. However, the mechanism of protein secretion via T3SSs is unknown. A characteristic of many effectors is that they require specific chaperones for efficient delivery via T3SSs; however, no chaperone has been experimentally identified in the T3SSs of V. parahaemolyticus. In this study, we identified candidate T3SS1-associated chaperones from genomic sequence data and examined their roles in effector secretion/translocation and binding to their cognate substrates. From these experiments, we concluded that there is a T3S-associated chaperone, VecA, for a cytotoxic T3SS1-dependent effector, VepA. Further analysis using pulldown and secretion assays characterized the chaperone-binding domain encompassing the first 30–100 amino acids and an amino terminal secretion signal encompassing the first 5–20 amino acids on VepA. These findings will provide a strategy to clarify how the T3SS1 of V. parahaemolyticus secretes its specific effectors.Keywords
This publication has 21 references indexed in Scilit:
- Identification of Two Translocon Proteins of Vibrio parahaemolyticus Type III Secretion System 2Infection and Immunity, 2008
- Vibrio parahaemolyticusInhibition of Rho Family GTPase Activation Requires a Functional Chromosome I Type III Secretion SystemInfection and Immunity, 2008
- Arp2/3-independent assembly of actin by Vibrio type III effector VopLProceedings of the National Academy of Sciences of the United States of America, 2007
- Type III Secretion Systems and DiseaseClinical Microbiology Reviews, 2007
- Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2Cellular Microbiology, 2007
- Global Dissemination of Vibrio parahaemolyticus Serotype O3:K6 and Its SerovariantsClinical Microbiology Reviews, 2007
- Subversion of actin dynamics by EPEC and EHECCurrent Opinion in Microbiology, 2006
- The chaperone binding domain of SopE inhibits transport via flagellar and SPI‐1 TTSS in the absence of InvBMolecular Microbiology, 2005
- Process of Protein Transport by the Type III Secretion SystemMicrobiology and Molecular Biology Reviews, 2004
- Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V choleraeThe Lancet, 2003