The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges
Open Access
- 12 August 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 391 (3), 317-322
- https://doi.org/10.1016/0014-5793(96)00771-5
Abstract
The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether‐bridging lanthionine and 3‐methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A‐FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.This publication has 39 references indexed in Scilit:
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