Teratocarcinoma cell adhesion: Identification of a cell-surface protein involved in calcium-dependent cell aggregation
- 1 February 1982
- journal article
- research article
- Published by Elsevier BV in Cell
- Vol. 28 (2), 217-224
- https://doi.org/10.1016/0092-8674(82)90339-7
Abstract
Teratocarcinoma cells have a Ca 2+-dependent cell-cell adhesion site (t-CDS) that is unique in being inactivated with trypsin in the absence of Ca 2+ but not in the presence of Ca 2+. Fab fragments of antibodies raised against teratocarcinoma F9 cells dissociated by treatment with trypsin and calcium (anti-TC-F9) inhibit the aggregation of teratocarcinoma cells mediated by t-CDS. This inhibitory effect of Fab is removed when anti-TC-F9 is absorbed with F9 cells treated with trypsin and calcium (TC-F9), but not when it is absorbed with F9 cells treated with trypsin and EGTA (TE-F9). Comparisons of cell-surface antigens reactive to anti-TC-F9 in TC-F9 cells with those in TE-F9 cells reveal that only one component, with an approximate molecular weight of 140,000 (p140), is detected specifically on the surface of TC-F9 cells. When TC-F9 cells are retrypsinized in the absence of Ca 2+, a substance with an approximate molecular weight of 34,000 (p34) is released that can neutralize the aggregation-inhibitory effect of the Fab. This p34 interferes with the immunoprecipitation of p140 with anti-TC-F9, suggesting that p34 is a tryptic fragment of p140. Anti-TC-F9 Fab causes the dissociation of the monolayers of teratocarcinoma cells. This effect is removed by absorption of the Fab with p34 as well as with TC-F9 cells, but not with TE-F9 cells. These results suggest that p140 is essential for the function of t-CDS, and that this is an actual cell-adhesion molecule active in the establishment of monolayers of teratocarcinoma cells.This publication has 33 references indexed in Scilit:
- Selective adhesion of embryonal carcinoma cells and differentiated cells by Ca2+-dependent sitesDevelopmental Biology, 1981
- Two distinct adhesion mechanisms in embryonic neural retina cells: II. An immunological analysisDevelopmental Biology, 1981
- Two chick embryonic adhesion systems: Molecular vs tissue specificityJournal of Supramolecular Structure and Cellular Biochemistry, 1981
- Divalent antibodies to mouse embryonal carcinoma cells inhibit compaction in the mouse embryoDevelopmental Biology, 1980
- Cell-Cell adhesion molecule: Identification of a glycoprotein relevant to the Ca2+-independent aggregation of chinese hamster fibroblastsCell, 1980
- CELL ASSOCIATION PATTERN IN AGGREGATES CONTROLLED BY MULTIPLE CELL-CELL ADHESION MECHANISMSDevelopment, Growth & Differentiation, 1980
- Identification of a cell surface glycoprotein involved in cell aggregation in d. discoideumCell, 1979
- Experimental manipulation of cell surface to affect cellular recognition mechanismsDevelopmental Biology, 1979
- Immunological detection of cell surface components related with aggregation of Chinese hamster and chick embryonic cellsDevelopmental Biology, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970