Apoptosis in colorectal tumour cells: Induction by the short chain fatty acids butyrate, propionate and acetate and by the bile salt deoxycholate
- 27 January 1995
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 60 (3), 400-406
- https://doi.org/10.1002/ijc.2910600322
Abstract
The short chain fatty acids acetate, propionate and butyrate are produced when dietary fibre is fermented by the colonic bacteria. We have previously shown that sodium butyrate induces apoptosis in 3 colorectal tumour cell lines. We have extended our study to 3 adenoma and 4 carcinoma cell lines and investigated whether propionate and acetate also induce apoptosis. All 3 short chain fatty acids induced apoptosis at physiological concentrations, but of the 3, butyrate was the most effective. Since these fatty acids are produced as a result of bacterial fermentation of dietary fibre, this may in part explain the correlation between a high-fibre diet and low colorectal cancer incidence. Sodium butyrate induced apoptosis in all 7 of the cell lines studied; however, 2 of the 4 carcinoma cell lines (PC/JW/F1 and S/KS/F1) were more resistant to butyrate-induced apoptosis than the 3 adenoma cell lines, suggesting that at least some carcinomas may evolve mechanisms to protect the cells from the induction of apoptosis. The bile acid deoxycholic acid has previously been reported as a possible tumour promoter in the large intestine and its levels are reduced by dietary fibre. Concentrations of between 10 nM and 0.1 mM had no effect on either the proliferation or apoptosis of colonic tumour cells in vitro. However, a significant induction of apoptosis was obtained at a concentration of 0.5 mM. These results may have significance for the aetiology of colorectal cancer.Keywords
This publication has 26 references indexed in Scilit:
- Multiple cell cycle access to the apoptotic death programme in human neuroblastoma cellsFEBS Letters, 1993
- Butyrate production from dietary fibre and protection against large bowel cancer in a rat model.Gut, 1993
- Dietary Intake of Fiber and Decreased Risk of Cancers of the Colon and Rectum: Evidence From the Combined Analysis of 13 Case-Control StudiesJNCI Journal of the National Cancer Institute, 1992
- Specific cytogenetic abnormalities and k‐ras mutation in two new human colorectal‐adenoma‐derived cell linesInternational Journal of Cancer, 1992
- Activation of Epstein–Barr virus latent genes protects human B cells from death by apoptosisNature, 1991
- Antibody-mediated targeting of differentiation inducers to tumor cells: Inhibition of colonic cancer cell growth in vitro and in vivo a preliminary noteBiochemical and Biophysical Research Communications, 1989
- Antibodies to CD3/T-cell receptor complex induce death by apoptosis in immature T cells in thymic culturesNature, 1989
- The isolation and characterization of colorectal epithelial cell lines at different stages in malignant transformation from familial polyposis coli patientsInternational Journal of Cancer, 1984
- Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activationNature, 1980