Widespread Collaboration of Isw2 and Sin3-Rpd3 Chromatin Remodeling Complexes in Transcriptional Repression
- 1 October 2001
- journal article
- Published by Taylor & Francis Ltd in Molecular and Cellular Biology
- Vol. 21 (19), 6450-60
- https://doi.org/10.1128/mcb.21.19.6450-6460.2001
Abstract
The yeast Isw2 chromatin remodeling complex functions in parallel with the Sin3-Rpd3 histone deacetylase complex to repress early meiotic genes upon recruitment by Ume6p. For many of these genes, the effect of an isw2 mutation is partially masked by a functional Sin3-Rpd3 complex. To identify the full range of genes repressed or activated by these factors and uncover hidden targets of Isw2-dependent regulation, we performed full genome expression analyses using cDNA microarrays. We find that the Isw2 complex functions mainly in repression of transcription in a parallel pathway with the Sin3-Rpd3 complex. In addition to Ume6 target genes, we find that many Ume6-independent genes are derepressed in mutants lacking functional Isw2 and Sin3-Rpd3 complexes. Conversely, we find that ume6 mutants, but not isw2 sin3 or isw2 rpd3 double mutants, have reduced fidelity of mitotic chromosome segregation, suggesting that one or more functions of Ume6p are independent of Sin3-Rpd3 and Isw2 complexes. Chromatin structure analyses of two nonmeiotic genes reveals increased DNase I sensitivity within their regulatory regions in an isw2 mutant, as seen previously for one meiotic locus. These data suggest that the Isw2 complex functions at Ume6-dependent and -independent loci to create DNase I-inaccessible chromatin structure by regulating the positioning or placement of nucleosomes.Keywords
This publication has 57 references indexed in Scilit:
- Improved Background Correction for Spotted DNA MicroarraysJournal of Computational Biology, 2002
- In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2pGenes & Development, 2001
- Functional Discovery via a Compendium of Expression ProfilesCell, 2000
- Purification and Characterization of a Human Factor That Assembles and Remodels ChromatinPublished by Elsevier BV ,2000
- The language of covalent histone modificationsNature, 2000
- Repression: Targeting the Heart of the MatterCell, 1999
- Fundamentally Different Logic of Gene Regulation in Eukaryotes and ProkaryotesCell, 1999
- Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic ScaleScience, 1997
- A complex containing N-CoR, mSln3 and histone deacetylase mediates transcriptional repressionNature, 1997
- Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure.Genes & Development, 1992