Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach: Size-Selective Sheltering of Catalase in Metal–Organic Framework Microcrystals
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- 27 March 2015
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 137 (13), 4276-4279
- https://doi.org/10.1021/ja513058h
Abstract
We develop a new concept to impart new functions to biocatalysts by combining enzymes and metal–organic frameworks (MOFs). The proof-of-concept design is demonstrated by embedding catalase molecules into uniformly sized ZIF-90 crystals via a de novo approach. We have carried out electron microscopy, X-ray diffraction, nitrogen sorption, electrophoresis, thermogravimetric analysis, and confocal microscopy to confirm that the ∼10 nm catalase molecules are embedded in 2 μm single-crystalline ZIF-90 crystals with ∼5 wt % loading. Because catalase is immobilized and sheltered by the ZIF-90 crystals, the composites show activity in hydrogen peroxide degradation even in the presence of protease proteinase K.Funding Information
- Boston College
- American Chemical Society Petroleum Research Fund
- Ministry of Science and Technology, Taiwan (101-2623-E-002-005-ET, 101-2628-E-002-015-MY3, 101-2923-E-002-012-MY3, 103-2113-M-008-001, 103-2218-E-002-101)
- Ministry of Education, Taiwan
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