Detection of ochratoxin A in meat, wheat and plasma by an enzyme-linked immunosorbent assay

Abstract

Chicken meat, wheat and porcine plasma spiked with various amounts of ochratoxin A were extracted with 0.5% metaphosphoric acid-chloroform (1 : 1), acetonitrile-water (3 : 1) and chloroform alone, respectively, and the resulting extracts were analyzed by an enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody specific to ochratoxin A. The detection limit of ochratoxin A in these samples was 1 ng/g in the present simplified ELISA.