Short DNA sequences inserted for gene targeting can accidentally interfere with off‐target gene expression
Open Access
- 22 January 2010
- journal article
- research article
- Published by Wiley in The FASEB Journal
- Vol. 24 (6), 1714-1724
- https://doi.org/10.1096/fj.09-140749
Abstract
Targeting of genes in mice, a key approach to study development and disease, often leaves a neo cassette, loxP, or FRT sites inserted in the mouse genome. Insertion of neo can influence the expressio...Funding Information
- Alexander von Humboldt-Stiftung
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