A link between the expression of the stem cell marker HMGA2, grading, and the fusion CRTC1‐MAML2 in mucoepidermoid carcinoma
- 11 June 2009
- journal article
- research article
- Published by Wiley in Genes, Chromosomes and Cancer
- Vol. 48 (9), 777-785
- https://doi.org/10.1002/gcc.20682
Abstract
Recently, the concept of cancer stem cells and their expression of embryonic stem cell markers has gained considerable experimental support. In this study, we examined the expression of one such marker, the high-mobility group AT-hook 2 gene (HMGA2) mRNA, in 53 formalin-fixed, paraffin-embedded mucoepidermoid carcinomas (MEC) and four normal parotid tissues using quantitative real-time RT-PCR (qPCR). MECs are often characterized by the fusion gene CRTC1-MAML2, the detection of which is an important tool for the diagnosis and prognosis of MEC. For detection of the CRTC1-MAML2 fusion transcript, we performed RT-PCR. The mean expression level of HMGA2 was higher in fusion negative (302.8 ± 124.4; n = 14) than in positive tumors (67.3 ± 13.1; n = 39). Furthermore, the fusion-negative tumors were often high-grade tumors and the HMGA2 expression level rose with the tumor grade (low: 43.7 ± 11.0, intermediate: 126.2 ± 28.3, and high: 271.2 ± 126.5). A significant difference was found in the HMGA2 expression levels between the different grading groups (one-way ANOVA, P = 0.04) and among the fusion-negative and -positive tumors (t-test, P = 0.05), indicating that the expression level of HMGA2 was closely linked to grading, the presence/absence of the CRTC1-MAML2 fusion, and the tumor behavior of MECs. These findings offer further evidence for the theory that the MEC group comprises two subgroups: one group with the CRTC1-MAML2 fusion, which is a group with a moderate aggressiveness and prognosis, and the other group lacking that fusion corresponding to an increased stemness, and thus, higher aggressiveness and worse prognosis.Keywords
This publication has 59 references indexed in Scilit:
- Treatment and survival outcomes based on histologic grading in patients with head and neck mucoepidermoid carcinomaCancer, 2008
- CRTC1/MAML2 fusion transcript in Warthin's tumor and mucoepidermoid carcinoma: Evidence for a common genetic associationGenes, Chromosomes and Cancer, 2008
- Alteration of β‐catenin localization in salivary pleomorphic adenomas is not related to t(3;8)(p21;q12) and is mainly present in non‐epithelial cell typesHistopathology, 2007
- Quantitative expression analysis in peripheral blood of patients with chronic myeloid leukaemia: Correlation betweenHMGA2expression and white blood cell countLeukemia & Lymphoma, 2007
- Sustained expression of Mect1-Maml2 is essential for tumor cell growth in salivary gland cancers carrying the t(11;19) translocationOncogene, 2006
- Mapping of candidate tumor suppressor genes on chromosome 12 in adenoid cystic carcinomaLaboratory Investigation, 2005
- Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalizationLaboratory Investigation, 2005
- Cytogenetic subtyping of 220 salivary gland pleomorphic adenomas: Correlation to occurrence, histological subtype, and in vitro cellular behaviorCancer Genetics and Cytogenetics, 1993
- Mucoepidermoid tumor of the parotid gland showing a translocation (3;8)(p21;q12) and a deletion (5)(q22) as sole chromosome abnormalitiesCancer Genetics and Cytogenetics, 1990
- Specificity of the involvement of chromosomes 8 and 12 in human mixed salivary‐gland tumoursJournal of Oral Pathology & Medicine, 1983