Mesenchymal Stem Cells Derived from Bone Marrow of Diabetic Patients Portrait Unique Markers Influenced by the Diabetic Microenvironment
- 1 January 2009
- journal article
- Published by JCFCorp SG PTE LTD in The Review of Diabetic Studies
- Vol. 6 (4), 260-270
- https://doi.org/10.1900/rds.2009.6.260
Abstract
Cellular microenvironment is known to play a critical role in the maintenance of human bone marrow-derived mesenchymal stem cells (BM-MSCs). It was uncertain whether BM-MSCs obtained from a 'diabetic milieu' (dBM-MSCs) offer the same regenerative potential as those obtained from healthy (non-diabetic) individuals (hBM-MSCs). To investigate the effect of diabetic microenvironment on human BM-MSCs, we isolated and characterized these cells from diabetic patients (dBM-MSCs). We found that dBM-MSCs expressed mesenchymal markers such as vimentin, smooth muscle actin, nestin, fibronectin, CD29, CD44, CD73, CD90, and CD105. These cells also exhibited multilineage differentiation potential, as evident from the generation of adipocytes, osteocytes, and chondrocytes when exposed to lineage specific differentiation media. Although the cells were similar to hBM-MSCs, 6% (3/54) of dBM-MSCs expressed proinsulin/C-peptide. Emanating from the diabetic microenvironmental milieu, we analyzed whether in vitro reprogramming could afford the maturation of the islet-like clusters (ICAs) derived from dBM-MSCs. Upon mimicking the diabetic hyperglycemic niche and the supplementation of fetal pancreatic extract, to differentiate dBM-MSCs into pancreatic lineage in vitro, we observed rapid differentiation and maturation of dBM-MSCs into islet-like cell aggregates. Thus, our study demonstrated that diabetic hyperglycemic microenvironmental milieu plays a major role in inducing the differentiation of human BM-MSCs in vivo and in vitro.Keywords
This publication has 48 references indexed in Scilit:
- Isolation and characterization of mesenchymal stem cells derived from bone marrow of patients with Parkinson’s diseaseIn Vitro Cellular & Developmental Biology – Animal, 2008
- Bone Marrow Mesenchymal Stem Cells from Healthy Donors and Sporadic Amyotrophic Lateral Sclerosis PatientsCell Transplantation, 2008
- Functional, molecular and proteomic characterisation of bone marrow mesenchymal stem cells in rheumatoid arthritisAnnals Of The Rheumatic Diseases, 2008
- Reversal of myofibroblasts by amniotic membrane stromal extractJournal of Cellular Physiology, 2008
- Mesenchymal Stem Cells Contribute to Insulin-Producing Cells Upon Microenvironmental Manipulation In VitroTransplantation Proceedings, 2007
- A Switch From Prohormone Convertase (PC)-2 to PC1/3 Expression in Transplanted α-Cells Is Accompanied by Differential Processing of Proglucagon and Improved Glucose Homeostasis in MiceDiabetes, 2007
- Phenotypic and Functional Comparison of Mesenchymal Stem Cells Derived from the Bone Marrow of Normal Adults and Patients with Hematologic Malignant DiseasesStem Cells and Development, 2007
- Biology of Incretins: GLP-1 and GIPGastroenterology, 2007
- Donor cell leukemia: insight into cancer stem cells and the stem cell nicheBlood, 2006
- Gata6 is an important regulator of mouse pancreas developmentDevelopmental Biology, 2006