HLA-G expression in human ovarian carcinoma counteracts NK cell function
Open Access
- 9 September 2007
- journal article
- research article
- Published by Elsevier BV in Annals Of Oncology
- Vol. 18 (11), 1804-1809
- https://doi.org/10.1093/annonc/mdm356
Abstract
Background: Human leukocyte antigen-G (HLA-G) is an important immunotolerant which could be a part of the strategies applied by malignant cells applied to avoid host immunosurveillance. Aberrant expression of HLA-G has been found in ovarian carcinoma. The aim of this study was to evaluate the HLA-G expression in ovarian cancer tissues and to explore its function in vitro. Materials and methods: HLA-G expression in 33 primary ovarian carcinoma tissues was analyzed using immunohistochemistry with the anti-HLA-G monoclonal antibody (mAb) 4H84. Furthermore, the function of HLA-G in NK cell cytotoxicity was determined in vitro by cloning and expression of HLA-G on the ovarian carcinoma cell OVCAR-3. Results: HLA-G expression was detected in 22/33 (66.7%) primary tumor tissues, but was absent in normal ovarian tissues (PPPConclusion: HLA-G was expressed in a significant number of primary ovarian carcinoma tissues, and HLA-G expression in OVCAR-3 could directly inhibit NK-92 cell lysis. Taken together, our results indicated that expression of HLA-G plays an important role in evasion of ovarian cancer cells from host immunosurveillance.Keywords
This publication has 24 references indexed in Scilit:
- Possible Roles of KIR2DL4 Expression on uNK Cells in Human PregnancyAmerican Journal of Reproductive Immunology, 2007
- Inhibition of term decidual NK cell cytotoxicity by soluble HLA‐G1American Journal of Reproductive Immunology, 2006
- HLA-G in human reproduction: aspects of genetics, function and pregnancy complicationsHuman Reproduction Update, 2005
- Tolerization of dendritic cells by HLA‐GEuropean Journal of Immunology, 2005
- Residues Met76 and Gln79 in HLA-G α1 domain involved in KIR2DL4 recognitionCell Research, 2005
- Membrane-bound HLA-G activates proliferation and interferon- production by uterine natural killer cellsMolecular Human Reproduction, 2004
- Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-GProceedings of the National Academy of Sciences of the United States of America, 2003
- Identification of HLA-G7 as a new splice variant of the HLA-G mRNA and expression of soluble HLA-G5, -G6, and -G7 transcripts in human transfected cellsHuman Immunology, 2000
- A Human Histocompatibility Leukocyte Antigen (HLA)-G–specific Receptor Expressed on All Natural Killer CellsThe Journal of Experimental Medicine, 1999
- A Class I Antigen, HLA-G, Expressed in Human TrophoblastsScience, 1990