The Glycosyltransferase Repertoire of the Spikemoss Selaginella moellendorffii and a Comparative Study of Its Cell Wall
Open Access
- 2 May 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (5), e35846
- https://doi.org/10.1371/journal.pone.0035846
Abstract
Spike mosses are among the most basal vascular plants, and one species, Selaginella moellendorffii, was recently selected for full genome sequencing by the Joint Genome Institute (JGI). Glycosyltransferases (GTs) are involved in many aspects of a plant life, including cell wall biosynthesis, protein glycosylation, primary and secondary metabolism. Here, we present a comparative study of the S. moellendorffii genome across 92 GT families and an additional family (DUF266) likely to include GTs. The study encompasses the moss Physcomitrella patens, a non-vascular land plant, while rice and Arabidopsis represent commelinid and non-commelinid seed plants. Analysis of the subset of GT-families particularly relevant to cell wall polysaccharide biosynthesis was complemented by a detailed analysis of S. moellendorffii cell walls. The S. moellendorffii cell wall contains many of the same components as seed plant cell walls, but appears to differ somewhat in its detailed architecture. The S. moellendorffii genome encodes fewer GTs (287 GTs including DUF266s) than the reference genomes. In a few families, notably GT51 and GT78, S. moellendorffii GTs have no higher plant orthologs, but in most families S. moellendorffii GTs have clear orthologies with Arabidopsis and rice. A gene naming convention of GTs is proposed which takes orthologies and GT-family membership into account. The evolutionary significance of apparently modern and ancient traits in S. moellendorffii is discussed, as is its use as a reference organism for functional annotation of GTs.Keywords
This publication has 107 references indexed in Scilit:
- AXY3 encodes a α-xylosidase that impacts the structure and accessibility of the hemicellulose xyloglucan in Arabidopsis plant cell wallsPlanta, 2010
- Identification of plant cell wall mutants by means of a forward chemical genetic approach using hydrolasesProceedings of the National Academy of Sciences of the United States of America, 2009
- A barley cellulose synthase-like CSLH gene mediates (1,3;1,4)-β- d -glucan synthesis in transgenic ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2009
- Protein structure prediction on the Web: a case study using the Phyre serverNature Protocols, 2009
- Independent origins of syringyl lignin in vascular plantsProceedings of the National Academy of Sciences of the United States of America, 2008
- Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2007
- Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thalianaProceedings of the National Academy of Sciences of the United States of America, 2007
- A gene from the cellulose synthase-like C family encodes a β-1,4 glucan synthaseProceedings of the National Academy of Sciences of the United States of America, 2007
- Genes for the peptidoglycan synthesis pathway are essential for chloroplast division in mossProceedings of the National Academy of Sciences of the United States of America, 2006
- Xyloglucans in the Primary Cell WallAnnual Review of Plant Physiology and Plant Molecular Biology, 1989