The Molecular Mechanisms of Traditional Chinese Medicine ZHENG Syndromes on Pancreatic Tumor Growth
Open Access
- 11 August 2010
- journal article
- research article
- Published by SAGE Publications in Integrative Cancer Therapies
- Vol. 9 (3), 291-297
- https://doi.org/10.1177/1534735410373922
Abstract
Background: Traditional Chinese medicine (TCM) syndromes ( ZHENG in Chinese) are the abstraction from the comprehensive analysis of clinical information gained by the four main diagnostic TCM methods: observation, listening, questioning, and pulse analyses. Proper TCM diagnosis is the most important principle to guide the prescribing of Chinese herbs. Objective: To evaluate the specific effect of TCM ZHENG on tumor growth and to examine the molecular mechanisms underlying ZHENG and tumor growth. Methods: The authors established subcutaneous tumor models of pancreatic cancer ZHENG syndromes of Damp heat ( Shi-Re) and Spleen deficiency ( Pi-Xu). Tissue samples of the subcutaneous transplanted tumors from each model were studied versus control tumors. CCR5 and CXCR4 proteins in these tissues were assayed by immunohistochemical staining. The expression of CCR5/CCL5/CCL4/CCL3 and CXCR4/SDF-1 mRNA was investigated by reverse transcriptase—polymerase chain reaction (RT-PCR). SDF-1, CCL4, CCL5, and CCL3, which are ligands of CXCR4 and CCR5, were examined by ELISA. Results: The study found that tumor models with different ZHENG were successfully established in each group; the tumor growth of Shi-Re group was slower than that of the control group. It was found that there was a significant difference in CCR5 mRNA expression levels among the Pi-Xu, Shi-Re, and control groups. The results of immunohistochemistry staining revealed that the positive rate of CCR5 protein in Shi-Re group, Pi-Xu group, and control group was 25.00%, 53.33%, 83.33%, respectively. The Shi-Re group expressed the lowest levels of CCL5 and CCL4. Conclusion: The results of the study suggest that the existence of TCM ZHENG may influence the tumor growth in pancreatic cancer, which might be mediated by the expression of CCR5/CCL5/CCL4. This finding may lead to the development of TCM ZHENG as a prognostic indicator in pancreatic tumor growth.Keywords
This publication has 18 references indexed in Scilit:
- The CCL5/CCR5 Axis in CancerPublished by Springer Science and Business Media LLC ,2009
- Phase II Trial of Curcumin in Patients with Advanced Pancreatic CancerClinical Cancer Research, 2008
- Functions of CXCL12 and CXCR4 in breast cancerCancer Letters, 2006
- Inflammatory chemokines in cancer growth and progressionEuropean Journal Of Cancer, 2006
- Expression of CCL5 (RANTES) and CCR5 in prostate cancerThe Prostate, 2005
- Are national quality standards for traditional Chinese herbal medicine sufficient?Complementary Therapies in Medicine, 2005
- Trafficking of Normal Stem Cells and Metastasis of Cancer Stem Cells Involve Similar Mechanisms: Pivotal Role of the SDF‐1–CXCR4 AxisThe International Journal of Cell Cloning, 2005
- Use of complementary and alternative medicine in cancer patients: a European surveyAnnals of Oncology, 2005
- Involvement of chemokine receptors in breast cancer metastasisNature, 2001
- THE DISTRIBUTION OF SECONDARY GROWTHS IN CANCER OF THE BREAST.The Lancet, 1889