Reexpression of RAG-1 and RAG-2 Genes in Activated Mature Mouse B cells
Open Access
- 20 December 1996
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 274 (5295), 2092-2094
- https://doi.org/10.1126/science.274.5295.2092
Abstract
Recombination activating genes (RAG-1 and RAG-2), involved in V(D)J rearrangement of immunoglobulin genes, have been thought to be expressed only in immature stages of B-cell development. However, RAG-1 and RAG-2 transcripts were found to be reexpressed in mature mouse B cells after culture with interleukin-4 in association with several different co-stimuli. Reexpression was also detected in draining lymph nodes from immunized mice. RAG-1 and RAG-2 proteins could be detected by immunofluorescence microscopy in the nuclei of B cells cultured in vitro and in the germinal centers of draining lymph nodes. These findings suggest that RAG gene products play a heretofore unsuspected role in mature B cells.Keywords
This publication has 21 references indexed in Scilit:
- Clonal selection and learning in the antibody systemNature, 1996
- Localization, interaction, and RNA binding properties of the V(D)J recombination-activating proteins RAG1 and RAG2Immunity, 1995
- New insights into V(D)J recombination and its role in the evolution of the immune systemImmunity, 1995
- Regulation of gene expression at early stages of B-cell differentiationCurrent Opinion in Immunology, 1994
- B lymphocytes may escape tolerance by revising their antigen receptors.The Journal of Experimental Medicine, 1993
- Restoration of T Cell Development in RAG-2-Deficient Mice by Functional TCR TransgenesScience, 1993
- Restricted expression of recombination activating gene (RAG‐1) in mouse lymphoid tissuesCell Biochemistry and Function, 1992
- RAG-1-deficient mice have no mature B and T lymphocytesCell, 1992
- Signal transduction through the human IL-2 receptor β-chain expressed in IL-6- dependent mouse B cell hybridomaInternational Immunology, 1991
- Peanut lectin binding properties of germinal centers of mouse lymphoid tissueNature, 1980