CONTRIBUTIONS OF DONOR CD4 AND CD8 CELLS TO LIVER INJURY DURING MURINE GRAFT-VERSUS-HOST DISEASE1
- 1 December 1996
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Transplantation
- Vol. 62 (11), 1621-1628
- https://doi.org/10.1097/00007890-199612150-00016
Abstract
We have determined the capacity of donor CD4 and CD8 T cells to mediate liver injury in the B10.D2 (donor) into BALB/c (host) chronic graft-versus-host disease (GVHD) model. First, we compared the effects of treating GVHD mice with anti-CD4 or anti-CD8 versus no treatment on the liver histology scores and elevated serum IgE levels in this model. We also examined the abilities of purified donor total T, CD4, and CD8 cells to mediate hepatic GVHD lesions. Anti-CD4 and anti-CD8 treatments caused profound depletion of peripheral CD4+ and CD8+ cells, respectively, and produced a relative enrichment of the CD8+ and CD4+ cells in the liver. Hepatic GVHD lesions and elevated serum IgE concentrations were both suppressed by anti-CD4 treatment. Anti-CD8 treatment had no effect on the severity of hepatic lesions and caused a significant increase in serum IgE levels. Attempts to induce hepatic GVHD with purified donor CD4 and CD8 cells were inconclusive because the onset of liver lesions was delayed and the lesions in both groups were contaminated by the opposite subset. Altogether, our results indicate that both hepatic lesions and elevated serum IgE concentrations in this GVHD model are dependent on donor CD4 cells. Donor CD4 cells mediated hepatic GVHD in the absence of CD8 cells. Donor CD8 cells did not produce hepatic GVHD in the absence of CD4 cells and appeared to be dependent on CD4 cells.Keywords
This publication has 19 references indexed in Scilit:
- T CELL SUBSETS INVOLVED IN LETHAL GRAFT-VERSUS-HOST DISEASE DIRECTED TO IMMUNODOMINANT MINOR HISTOCOMPATIBILITY ANTIGENSTransplantation, 1994
- T CELL SUBSETS REQUIRED FOR IN VIVO AND IN VITRO RESPONSES TO SINGLE AND MULTIPLE MINOR HISTOCOMPATIBILITY ANTIGENSTransplantation, 1992
- Suppressor function of hepatic mononuclear inflammatory cells during murine chronic graft-vs-host disease: I. Macrophage-enriched cells mediate suppression in the liverCellular Immunology, 1991
- Graft-versus-host disease and animal models for sclerodermaCurrent Opinion in Rheumatology, 1990
- Immunoglobulin dysregulation in murine graft-vs-host disease: A hyper-IgE syndromeClinical Immunology and Immunopathology, 1990
- ADOPTIVE IMMUNITY IN IMMUNE-DEFICIENT scid/scid MICETransplantation, 1988
- Variable capacity of L3T4+ T cells to cause lethal graft-versus-host disease across minor histocompatibility barriers in mice.The Journal of Experimental Medicine, 1987
- Identification of interleukin 2-producing T helper cells within murine Lyt-2+ T lymphocytes: frequency, specificity and clonal segregation from Lyt-2+ precursors of cytotoxic T lymphocytesEuropean Journal of Immunology, 1987
- SKIN ALLOGRAFT REJECTION BY L3/t4+ AND LYT-2+ T CELL SUBESTSTransplantation, 1986
- Chronic graft-versus-host disease as a model for scleroderma: II. Mast cell depletion with deposition of immunoglobulins in the skin and fibrosisCellular Immunology, 1985