Disruption of OsYSL15 Leads to Iron Inefficiency in Rice Plants
Open Access
- 31 May 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 150 (2), 786-800
- https://doi.org/10.1104/pp.109.135418
Abstract
Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe3+; these complexes are then transported across the plasma membrane. We have characterized OsYSL15, one of the rice (Oryza sativa) YS1-like (YSL) genes that are strongly induced by iron (Fe) deficiency. The OsYSL15 promoter fusion to beta-glucuronidase showed that it was expressed in all root tissues when Fe was limited. In low-Fe leaves, the promoter became active in all tissues except epidermal cells. This activity was also detected in flowers and seeds. The OsYSL15: green fluorescent protein fusion was localized to the plasma membrane. OsYSL15 functionally complemented yeast strains defective in Fe uptake on media containing Fe3+-deoxymugineic acid and Fe2+-nicotianamine. Two insertional osysl15 mutants exhibited chlorotic phenotypes under Fe deficiency and had reduced Fe concentrations in their shoots, roots, and seeds. Nitric oxide treatment reversed this chlorosis under Fe-limiting conditions. Overexpression of OsYSL15 increased the Fe concentration in leaves and seeds from transgenic plants. Altogether, these results demonstrate roles for OsYSL15 in Fe uptake and distribution in rice plants.This publication has 64 references indexed in Scilit:
- Nitric oxide accumulation is required for molecular and physiological responses to iron deficiency in tomato rootsThe Plant Journal, 2007
- Rice Immature Pollen 1 ( RIP1 ) is a Regulator of Late Pollen DevelopmentPlant and Cell Physiology, 2006
- Mutations in Arabidopsis Yellow Stripe-Like1 and Yellow Stripe-Like3 Reveal Their Roles in Metal Ion Homeostasis and Loading of Metal Ions in SeedsPlant Physiology, 2006
- Generation of a flanking sequence‐tag database for activation‐tagging lines in japonica riceThe Plant Journal, 2005
- RiceUndeveloped Tapetum1Is a Major Regulator of Early Tapetum DevelopmentPlant Cell, 2005
- Nitric Oxide Improves Internal Iron Availability in PlantsPlant Physiology, 2002
- Identification of a Signal That Distinguishes between the Chloroplast Outer Envelope Membrane and the Endomembrane System in VivoPlant Cell, 2001
- Trafficking of Phosphatidylinositol 3-Phosphate from the trans-Golgi Network to the Lumen of the Central Vacuole in Plant CellsPlant Cell, 2001
- Specific Fe2+uptake system in strategy I plants inducible under Fe deficiencyJournal of Plant Nutrition, 2000
- Effect of iron deficiency on S‐adenosylmethionine synthetase in barley rootsJournal of Plant Nutrition, 1996