Silicon enhances suberization and lignification in roots of rice (Oryza sativa)
Open Access
- 13 December 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 62 (6), 2001-2011
- https://doi.org/10.1093/jxb/erq392
Abstract
The beneficial element silicon (Si) may affect radial oxygen loss (ROL) of rice roots depending on suberization of the exodermis and lignification of sclerenchyma. Thus, the effect of Si nutrition on the oxidation power of rice roots, suberization and lignification was examined. In addition, Si-induced alterations of the transcript levels of 265 genes related to suberin and lignin synthesis were studied by custom-made microarray and quantitative Real Time-PCR. Without Si supply, the oxidation zone of 12 cm long adventitious roots extended along the entire root length but with Si supply the oxidation zone was restricted to 5 cm behind the root tip. This pattern coincided with enhanced suberization of the exodermis and lignification of sclerenchyma by Si supply. Suberization of the exodermis started, with and without Si supply, at 4–5 cm and 8–9 cm distance from the root tip (drt), respectively. Si significantly increased transcript abundance of 12 genes, while two genes had a reduced transcript level. A gene coding for a leucine-rich repeat protein exhibited a 25-fold higher transcript level with Si nutrition. Physiological, histochemical, and molecular-biological data showing that Si has an active impact on rice root anatomy and gene transcription is presented here.Keywords
This publication has 52 references indexed in Scilit:
- Measurements of oxygen permeability coefficients of rice (Oryza sativa L.) roots using a new perfusion techniqueJournal of Experimental Botany, 2008
- Suberin — a biopolyester forming apoplastic plant interfacesCurrent Opinion in Plant Biology, 2007
- Soybean Root Suberin: Anatomical Distribution, Chemical Composition, and Relationship to Partial Resistance to Phytophthora sojaePlant Physiology, 2007
- A Genomic Approach to Suberin Biosynthesis and Cork DifferentiationPlant Physiology, 2007
- Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCRBiochemical and Biophysical Research Communications, 2006
- Long‐distance transport of gases in plants: a perspective on internal aeration and radial oxygen loss from rootsPlant, Cell & Environment, 2003
- Gene Expression Omnibus: NCBI gene expression and hybridization array data repositoryNucleic Acids Research, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Airspace structure and mathematical modelling of oxygen diffusion, aeration and anoxia in Eleocharis sphacelata R. Br. RootsMarine and Freshwater Research, 1994
- Radial Oxygen Losses from Intact Rice Roots as Affected by Distance from the Apex, Respiration and WaterloggingPhysiologia Plantarum, 1971