Small-Animal Whole-Body Photoacoustic Tomography: A Review
- 25 September 2013
- journal article
- review article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Biomedical Engineering
- Vol. 61 (5), 1380-1389
- https://doi.org/10.1109/tbme.2013.2283507
Abstract
With the wide use of small animals for biomedical studies, in vivo small-animal whole-body imaging plays an increasingly important role. Photoacoustic tomography (PAT) is an emerging whole-body imaging modality that shows great potential for preclinical research. As a hybrid technique, PAT is based on the acoustic detection of optical absorption from either endogenous tissue chromophores, such as oxyhemoglobin and deoxyhemoglobin, or exogenous contrast agents. Because ultrasound scatters much less than light in tissue, PAT generates high-resolution images in both the optical ballistic and diffusive regimes. Using near-infrared light, which has relatively low blood absorption, PAT can image through the whole body of small animals with acoustically defined spatial resolution. Anatomical and vascular structures are imaged with endogenous hemoglobin contrast, while functional and molecular images are enabled by the wide choice of exogenous optical contrasts. This paper reviews the rapidly growing field of small-animal whole-body PAT and highlights studies done in the past decade.Keywords
Funding Information
- National Institutes of Health (DP1 EB016986, R01 EB016963, R01 EB008085, R01CA134539, U54CA136398, R01 EB010049, R01CA157277, R01CA159959)
- Microphotoacoustics, Inc., and Endra, Inc.
This publication has 77 references indexed in Scilit:
- Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivoNeuroImage, 2013
- Investigation of iterative image reconstruction in three-dimensional optoacoustic tomographyPhysics in Medicine & Biology, 2012
- Deep‐Tissue Photoacoustic Tomography of a Genetically Encoded Near‐Infrared Fluorescent ProbeAngewandte Chemie-International Edition, 2011
- Three-dimensional photoacoustic tomography based on the focal-line conceptJournal of Biomedical Optics, 2011
- Bright and stable near-infrared fluorescent protein for in vivo imagingNature Biotechnology, 2011
- Photoacoustic Imaging With a Commercial Ultrasound System and a Custom ProbeJapanese Journal of Clinical Oncology, 2011
- Label-free oxygen-metabolic photoacoustic microscopy in vivoJournal of Biomedical Optics, 2011
- Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applicationsJournal of Photochemistry and Photobiology B: Biology, 2009
- Multiscale photoacoustic microscopy and computed tomographyNature Photonics, 2009
- Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer modelsAnalytical Biochemistry, 2008