An immunofluorescent method using crithidia luciliae to detect antibodies to double‐stranded DNA
Open Access
- 1 April 1977
- journal article
- research article
- Published by Wiley in Arthritis & Rheumatism
- Vol. 20 (3), 811-814
- https://doi.org/10.1002/art.1780200308
Abstract
The immunochemical specificity of the immunofluorescent Crithidia luciliae method for detection of antibodies to double-stranded DNA (dsDNA) was confirmed by demonstrating abolition of staining by DNase digestion and by absorption with dsDNA. This method was less sensitive than a Millipore filter method for detecting antibodies to DNA. It was positive only in subjects with systemic lupus erythematosus or drug-induced antinuclear factors. This technique appears suitable for study of the immunochemical characteristics of antibodies to dsDNA.This publication has 12 references indexed in Scilit:
- Physicochemical properties of kinetoplast DNA from Crithidia acanthocephali. Crithidia luciliae, and Trypanosoma lewisi.The Journal of cell biology, 1975
- IMMUNOLOGY OF DNA. III. CRITHIDIA LUCILIAE, A SIMPLE SUBSTRATE FOR THE DETERMINATION OF ANTI‐dsDNA WITH THE IMMUNOFLUORESCENCE TECHNIQUE*Annals of the New York Academy of Sciences, 1975
- A millipore filter assay for antibodies to native dna in sera of patients with systemic lupus erythematosusArthritis & Rheumatism, 1973
- SIGNIFICANCE OF ANTI-D.N.A. ANTIBODIES IN SYSTEMIC LUPUS ERYTHEMATOSUSThe Lancet, 1971
- Kinetoplast DNA. A unique macromolecular structure of considerable size and mechanical resistanceBiochemical and Biophysical Research Communications, 1971
- Measurement of Serum DNA-Binding Activity in Systemic Lupus ErythematosusNew England Journal of Medicine, 1969
- ELECTRON MICROSCOPY OF THE CIRCULAR KINETOPLASTIC DNA FROM TRYPANOSOMA CRUZI: OCCURRENCE OF CATENATED FORMSProceedings of the National Academy of Sciences of the United States of America, 1969
- L'absence d'histone dans le kinétonucléus des trypanosomes: Etude cytochimiqueExperimental Cell Research, 1965
- Significance of antibody to DNA in systemic lupus erythematosusArthritis & Rheumatism, 1964
- A method for the detection of RNA-DNA complexesBiochemical and Biophysical Research Communications, 1963