fimA Genotypes and Multilocus Sequence Types of Porphyromonas gingivalis from Patients with Periodontitis
- 1 January 2008
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 46 (1), 31-42
- https://doi.org/10.1128/jcm.00986-07
Abstract
Fimbriae are important virulence factors of pathogenic bacteria, facilitating their attachment to host and bacterial cells. In the periodontal pathogen Porphyromonas gingivalis , the fimA gene is classified into six types (genotypes I, Ib, II, III, IV, and V) on the basis of different nucleotide sequences, with fimA genotypes II and IV being prevalent in isolates from patients with periodontitis. The aims of this study were to examine the distribution of fimA genotypes in a collection of 82 P. gingivalis isolates from adult periodontitis patients of worldwide origin and to investigate the relationship between the fimA genotypes and the sequence types (STs), as determined by multilocus sequence typing (MLST), of the isolates. The fimA gene was amplified by PCR with primer sets specific for each genotype. The STs of all strains were assigned according to the MLST database for P. gingivalis ( www.pubmlst.org/pgingivalis ). The 82 strains showed extensive genetic diversity and were assigned to 69 STs. Only isolates with closely related STs harbored the same fimA genotype. Twenty-eight (34.1%) strains harbored fimA genotype II, while only the reference strain for fimA genotype V reacted with the primers specific for this genotype. Twenty-one isolates (25.6%) were positive by more than one of the fimA PCR assays; the most frequent combinations were genotypes I, Ib, and II (eight isolates) and genotypes I and II (four isolates). Sequencing of the fimA gene from selected isolates did not support the observed specific fimA genotype combinations, suggesting that the genotyping method used for the major fimbriae in P. gingivalis should be reevaluated.Keywords
This publication has 46 references indexed in Scilit:
- Adhesion and invasion to epithelial cells by fimA genotypes of Porphyromonas gingivalisOral Microbiology and Immunology, 2006
- The etiology of periodontal disease revisited by population genetic analysisPeriodontology 2000, 2006
- Protein structure prediction servers at University College LondonNucleic Acids Research, 2005
- Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the ‘red complex’, a prototype polybacterial pathogenic consortium in periodontitisPeriodontology 2000, 2005
- The prevalence and pathogenic differences of Porphyromonas gingivalis fimA genotypes in patients with aggressive periodontitisJournal of Periodontal Research, 2005
- Distribution of fimA genotypes of Porphyromonas gingivalis in subjects with various periodontal conditionsOral Microbiology and Immunology, 2004
- Obesity and Periodontal Disease in Young, Middle‐Aged, and Older AdultsThe Journal of Periodontology, 2003
- Prevalence of Specific Genotypes of Porphyromonas gingivalis fimA and Periodontal Health StatusJournal of Dental Research, 2000
- Molecular Cloning and Sequencing of the Fimbrilin Gene of Porphyromonas gingivalis Strains and Characterization of Recombinant ProteinsBiochemical and Biophysical Research Communications, 1993
- How clonal are bacteria?Proceedings of the National Academy of Sciences of the United States of America, 1993