Analysis of trace residues of tetracyclines in animal tissues and fluids using metal chelate affinity chromatography/HPLC
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis Ltd in Food Additives & Contaminants
- Vol. 8 (1), 55-64
- https://doi.org/10.1080/02652039109373955
Abstract
A novel method of analysis for the trace residue determination of tetracyclines in animal tissues and fluids has been developed. Clean‐up of sample extracts is based upon the specific ability of tetracyclines to chelate with divalent metal ions (metal chelate affinity chromatography, MCAC) and determination made by high‐performance liquid chromatography. The method has been tested for the determination of oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) in porcine kidney and muscle, ovine kidney, bovine kidney and milk, and trout muscle. Recoveries at the 0.05 mg/kg level for OTC, TC and CTC respectively were 75%, 63%, 73% in porcine kidney, 77%, 79%, 76% in porcine muscle, 85%, 54%, 53% in bovine kidney, 78%, 63%, 57% in ovine kidney, 75%, 58%, 56% in fish (trout) muscle, and 80%, 59%, 59% in bovine milk. At this level both within‐ and between‐batch precision, as measured by the coefficient of variation (CV), was less than 10%. Determination to the 0.01 mg/kg level was carried out in all cases, although the method becomes less precise. The method has been used for several months and found to be both reliable and sufficiently rapid for use as a routine quantitative screening procedure. When coupled with liquid chromatography‐mass spectrometry (LC‐MS) it is suitable for use as a confirmatory method. Analysis of animals treated with tetracyclines has been carried out.Keywords
This publication has 10 references indexed in Scilit:
- Development of a method for the determination of oxytetracycline in troutFood Additives & Contaminants, 1988
- Improvement of chemical analysis of antibiotics : XIII. Systematic simultaneous analysis of residual tetracyclines in animal tissues using thin-layer and high-performance liquid chromatographyJournal of Chromatography A, 1987
- Determination of tetracycline antibiotics in tissues and blood serum of cattle and swine by high-performance liquid chromatographyJournal of Chromatography A, 1986
- Effect of the silica support of bonded reversed-phase columns on chromatography of some antibiotic compoundsJournal of Chromatography A, 1986
- High-performance liquid chromatography of tetracyclinesJournal of Chromatography A, 1986
- Improvement of chemical analysis of antibiotics : VIII. Application of prepacked C18 cartridge for the analysis of tetracycline residues in animal liverJournal of Chromatography A, 1985
- Rapid, sensitive and specific determination of oxytetracycline residues in bovine milk and meat by CAD MIKES analysis at the 1 ppb levelJournal of Mass Spectrometry, 1985
- Simultaneous determination of antibiotics and synthetic antibacterials in fish by high performance liquid chromatography.Eisei kagaku, 1985
- Improved high-performance liquid chromatographic procedure for the determination of tetracyclines in plasma, urine and tissues.Journal of Chromatography A, 1978
- Determination of Tetracyclines by Extraction of Fluorescent Complexes. Application to Biological MaterialsAnalytical Chemistry, 1961