Low-Density Lipoprotein Cholesterol Determines Oxidative Stress and Endothelial Dysfunction in Saphenous Veins From Patients With Coronary Artery Disease
- 1 January 2006
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 26 (1), 218-223
- https://doi.org/10.1161/01.atv.0000193626.22269.45
Abstract
Objective— There is evidence for a relationship between endothelial dysfunction and cardiovascular disease, but a causative role for oxidative stress remains to be determined. Methods and Results— We studied 188 patients with severe coronary artery disease (CAD), of whom 51 were age and sex matched with 51 healthy controls undergoing varicose vein surgery. Relaxation of saphenous vein to calcium ionophore, apocynin, and allopurinol was studied together with the markers of oxidative stress, total antioxidant capacity and reduced/oxidized glutathione ratio. Vascular superoxide levels were measured using lucigenin chemiluminescence and hydroethidine. Relaxation to calcium ionophore was decreased in CAD compared with control patients (maximum relaxation 26±2% versus 60±1%; P P =0.008), whereas superoxide inhibition with apocynin or allopurinol had a greater effect on vasorelaxation in CAD patients. Low-density lipoprotein (LDL) cholesterol predicted relaxation to calcium ionophore ( P P Conclusions— Endothelial dysfunction is associated with raised levels of superoxide and biomarkers of oxidative stress in saphenous veins from CAD patients. LDL cholesterol is a major determinant of endothelial dysfunction and oxidative stress in these patients. These results support intensive LDL cholesterol-lowering therapy as suggested by recent clinical trials.Keywords
This publication has 22 references indexed in Scilit:
- Therapeutic potential of nitric oxide donors in the prevention and treatment of atherosclerosisEuropean Heart Journal, 2005
- Reactive oxygen species in vascular biology: implications in hypertensionHistochemistry and Cell Biology, 2004
- Reactive Oxygen Species in the VasculatureHypertension, 2003
- The vascular NAD(P)H oxidases as therapeutic targets in cardiovascular diseasesTrends in Pharmacological Sciences, 2003
- Antioxidant Activities and Oxidative Stress Byproducts in Human HypertensionHypertension, 2003
- Novel NAD(P)H Oxidase Inhibitor Suppresses Angioplasty-Induced Superoxide and Neointimal Hyperplasia of Rat Carotid ArteryCirculation Research, 2003
- Role of oxidative stress in atherosclerosisThe American Journal of Cardiology, 2003
- The Overexpression Catalase Reduces NO‐Mediated Inhibition of Endothelial NO SynthaseIUBMB Life, 2002
- Vascular Release of Superoxide Radicals Is Enhanced in Hypercholesterolemic RabbitsJournal of Cardiovascular Pharmacology, 1994
- Impaired endothelium-dependent vasodilation of forearm resistance vessels in hypercholesterolaemiaThe Lancet, 1992