Protective Effect of Total Flavones of Abelmoschus manihot L. Medic Against Poststroke Depression Injury in Mice and Its Action Mechanism
Open Access
- 26 February 2009
- journal article
- research article
- Published by Wiley in The Anatomical Record
- Vol. 292 (3), 412-422
- https://doi.org/10.1002/ar.20864
Abstract
Total flavones of Abelmoschus manihot L. Medic (TFA) is the major active component isolated from the traditional Chinese herb Abelmoschus manihot L. Medic. We investigated the protective effect of TFA against poststroke depression (PSD) injury in mice and its action mechanism. A mouse model of PSD was induced by middle cerebral artery occlusion (MACO) 30 min/reperfusion, followed by isolation feeding and chronic unpredictable mild stress for 2 weeks. Treatment groups received TFA at three different doses (160, 80, and 40 mg/kg, p.o.) or fluoxetine (Flu, 2.5 mg/kg, p.o.) daily for 24 days. Change in behavior, brain tissue malondialdehyde (MDA) levels, and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. The expression of brain-derived neurotrophic factor (BDNF) was detected by immunohistochemistry, and mRNA expression of BDNF and cAMP response element-binding protein (CREB) analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Treatment with TFA (160, 80, and 40 mg/kg) significantly ameliorated mice escape-directed behavioral impairment induced by PSD, markedly reduced MDA levels, and increased the activity of SOD, GSH-Px close to normal levels. TFA administration also attenuated PSD-induced neuronal death/losses, upregulated expression of BDNF both at mRNA and protein levels, as well as CREB mRNA levels. TFA had a protective effect against PSD injury in mice. Cardioprotection involves the inhibition of lipid peroxidation and upregulation of BDNF-CREB levels in the hippocampus, which may also be important mechanism of its antidepressants. This potential protection makes TFA a promising therapeutic agent for the PSD. Anat Rec, 292:412–422, 2009.Keywords
This publication has 48 references indexed in Scilit:
- Chronic fluoxetine administration inhibits extracellular signal‐regulated kinase 1/2 phosphorylation in rat brainJournal of Neurochemistry, 2005
- P.1.11 Selective effects of antidepressants on CREB phosphorylation and related kinase cascadeEuropean Neuropsychopharmacology, 2004
- Effects of Recombinant Tissue Plasminogen Activator After Intraluminal Thread Occlusion in Mice: Role of Hemodynamic AlterationsStroke, 2001
- Treatment of Cognitive Impairment After Poststroke DepressionStroke, 2000
- Poststroke depression correlates with cognitive impairment and neurological deficitsStroke, 1999
- Mechanisms that Result in Damage During and Following Cerebral IschemiaNeuroscience & Biobehavioral Reviews, 1997
- Oxidative damage in Alzheimer'sNature, 1996
- Physiology of the NeurotrophinsAnnual Review of Neuroscience, 1996
- Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke, 1989
- Experimental ischemic stroke: a review.Stroke, 1984