A Microsatellite Linkage Map of Barramundi, Lates calcarifer
- 1 February 2007
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 175 (2), 907-915
- https://doi.org/10.1534/genetics.106.059972
Abstract
Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its compact genome (∼700 Mb) is among the smallest genomes of food fish species. We established a first-generation genetic linkage map of Barramundi with a mapping panel containing three parents (two males and one female) and 93 progeny. A total of 240 microsatellite markers were mapped into 24 linkage groups. Among these markers, 10 were located in ESTs and known genes. The total lengths of the female and male maps were 873.8 and 414.5 cM with an average marker spacing of 6.20 and 4.70 cM, respectively. Comparing the flanking sequences of the 240 Barramundi microsatellites with the assembled whole-genome sequences of Tetraodon nigrovidiris revealed 55 homologous sequences located in 19 of the 21 chromosomes of T. nigrovidiris. The map will not only enable the mapping of quantitative trait loci, but also provide new resources for understanding the evolution of fish genomes.Keywords
This publication has 36 references indexed in Scilit:
- Genetic analyses of Asian seabass stocks using novel polymorphic microsatellitesAquaculture, 2006
- Characterization of two parvalbumin genes and their association with growth traits in Asian seabass (Lates calcarifer)Animal Genetics, 2006
- The Complete Mitochondrial Genome Sequence and Characterization of Single-Nucleotide Polymorphisms in the Control Region of the Asian Seabass (Lates calcarifer)Marine Biotechnology, 2006
- A simple and affordable method for high‐throughput DNA extraction from animal tissues for polymerase chain reactionElectrophoresis, 2005
- Genetic linkage maps of two yellowtails (Seriola quinqueradiata and Seriola lalandi)Aquaculture, 2004
- Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotypeNature, 2004
- A Medaka Gene Map: The Trace of Ancestral Vertebrate Proto-Chromosomes Revealed by Comparative Gene MappingGenome Research, 2004
- Adaptive evolution and explosive speciation: the cichlid fish modelNature Reviews Genetics, 2004
- A genetic linkage map of common carp (Cyprinus carpio L.) And mapping of a locus associated with cold toleranceAquaculture, 2003
- Characterization of Microsatellites in the IGF-2 and GH Genes of Asian Seabass (Lates calcarifer)Marine Biotechnology, 2001