Stromal stem cells from adipose tissue and bone marrow of age‐matched female donors display distinct immunophenotypic profiles
- 20 September 2010
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 226 (3), 843-851
- https://doi.org/10.1002/jcp.22408
Abstract
Adipose tissue is composed of lipid‐filled mature adipocytes and a heterogeneous stromal vascular fraction (SVF) population of cells. Similarly, the bone marrow (BM) is composed of multiple cell types including adipocytes, hematopoietic, osteoprogenitor, and stromal cells necessary to support hematopoiesis. Both adipose and BM contain a population of mesenchymal stromal/stem cells with the potential to differentiate into multiple lineages, including adipogenic, chondrogenic, and osteogenic cells, depending on the culture conditions. In this study we have shown that human adipose‐derived stem cells (ASCs) and bone marrow mesenchymal stem cells (BMSCs) populations display a common expression profile for many surface antigens, including CD29, CD49c, CD147, CD166, and HLA‐abc. Nevertheless, significant differences were noted in the expression of CD34 and its related protein, PODXL, CD36, CD 49f, CD106, and CD146. Furthermore, ASCs displayed more pronounced adipogenic differentiation capability relative to BMSC based on Oil Red staining (7‐fold vs. 2.85‐fold induction). In contrast, no difference between the stem cell types was detected for osteogenic differentiation based on Alizarin Red staining. Analysis by RT‐PCR demonstrated that both the ASC and BMSC differentiated adipocytes and osteoblast displayed a significant upregulation of lineage‐specific mRNAs relative to the undifferentiated cell populations; no significant differences in fold mRNA induction was noted between ASCs and BMSCs. In conclusion, these results demonstrate human ASCs and BMSCs display distinct immunophenotypes based on surface positivity and expression intensity as well as differences in adipogenic differentiation. The findings support the use of both human ASCs and BMSCs for clinical regenerative medicine. J. Cell. Physiol. 226: 843–851, 2011.Keywords
This publication has 59 references indexed in Scilit:
- SPARC Inhibits Adipogenesis by Its Enhancement of β-Catenin SignalingPublished by Elsevier BV ,2009
- Adipose-derived stem cells: Isolation, expansion and differentiationMethods, 2008
- Circadian mechanisms in murine and human bone marrow mesenchymal stem cells following dexamethasone exposureBone, 2008
- Food entrainment of circadian gene expression altered in PPARα−/− brown fat and heartBiochemical and Biophysical Research Communications, 2007
- Playing with bone and fatJournal of Cellular Biochemistry, 2006
- Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statementCytotherapy, 2006
- Adult bone marrow–derived cells: Regenerative potential, plasticity, and tissue commitmentBasic Research in Cardiology, 2005
- Yield of human adipose-derived adult stem cells from liposuction aspiratesCytotherapy, 2004
- Surface protein characterization of human adipose tissue‐derived stromal cellsJournal of Cellular Physiology, 2001
- Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering, 2001