Cadherin/Catenin Complexes in Murine Epidermal Keratinocytes: E-Cadherin Complexes Containing either β-Catenin or Plakoglobin Contribute to Stable Cell-Cell Contacts
- 1 January 1998
- journal article
- research article
- Published by Taylor & Francis Ltd in Cell Adhesion and Communication
- Vol. 6 (1), 51-67
- https://doi.org/10.3109/15419069809069760
Abstract
The cadherin/catenin complexes expressed by a murine epidermal keratinocyte cell line PDV, expressing E- and P-cadherin, have been analysed using a combination of biochemical and confocal microscopy analysis. Two types of E-cadherin complexes, containing beta-catenin or plakoglobin and alpha-catenin, were detected in PDV cells as in other cell types, while P-cadherin was mainly detected in complexes containing beta-catenin and alpha-catenin in PDV and other murine epidermal keratinocytes. Biotin-labelling studies have shown that both types of E-cadherin complexes are present at the surface of confluent cells. Furthermore, confocal microscopy analysis indicated that E-cadherin/plakoglobin complexes are located in stable cell-cell contacts at the middle lateral membranes and associated with alpha-catenin and the actin cytoskeleton, with a similar distribution to that to the E-cadherin/beta-catenin complexes. In addition, E-cadherin/plakoglobin complexes not associated with alpha-catenin or the actin cytoskeleton were detected in lower planes of the lateral contacting membranes as well as E-cadherin non-associated with catenins in the more basal planes. These studies support that in murine epidermal keratinocytes both beta-catenin- and plakoglobin-containing E-cadherin complexes contribute to the maintenance of stable cell-cell contacts and suggest a differential role of the plakoglobin containing complexes in different epithelial cell types.Keywords
This publication has 39 references indexed in Scilit:
- Desmosomal Cadherin Binding Domains of PlakoglobinPublished by Elsevier BV ,1996
- Identification of Plakoglobin Domains Required for Association with N-cadherin and α-CateninPublished by Elsevier BV ,1995
- Delayed Assembly of Desmosomes in Keratinocytes with Disrupted Classic-Cadherin-Mediated Cell Adhesion by a Dominant Negative MutantJournal of Investigative Dermatology, 1995
- Distinct cadherin—catenin complexes in Ca2+ dependent cell—cell adhesionFEBS Letters, 1994
- Identification of the plakoglobin-binding domain in desmoglein and its role in plaque assembly and intermediate filament anchorage.The Journal of cell biology, 1994
- Dynamics of cadherin/catenin complex formation: novel protein interactions and pathways of complex assembly.The Journal of cell biology, 1994
- P-Cadherin and E-Cadherin Are Co-expressed in MDCK CellsBiochemical and Biophysical Research Communications, 1993
- Regulation of keratinocyte intercellular junction organization and epidermal morphogenesis by E-cadherinThe Journal of cell biology, 1992
- Comparison of ras activation during epidermal carcinogenesis in vitro and in vivoCarcinogenesis: Integrative Cancer Research, 1991
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970