Correspondence Between a Mammalian Spliceosome Component and an Essential Yeast Splicing Factor
- 1 October 1993
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 262 (5130), 105-108
- https://doi.org/10.1126/science.8211113
Abstract
None of the mammalian splicing factors that have been cloned corresponds to the yeast pre-messenger RNA splicing factors, the PRP proteins. Here, a generalizable strategy was used to isolate a complementary DNA encoding the mammalian spliceosome-associated protein (SAP) SAP 62. It is demonstrated that SAP 62 is the likely functional homolog of the yeast PRP11 protein. Both PRP11 and SAP 62 associate stably with the spliceosome, contain a single zinc finger, and display significant amino acid sequence similarity. Unlike PRP11, SAP 62 contains 22 proline-rich heptapeptide repeats at the carboxyl-terminus.Keywords
This publication has 35 references indexed in Scilit:
- Interaction of Mammalian Splicing Factor SF3a with U2 snRNP and Relation of Its 60-kD Subunit to Yeast PRP9Science, 1993
- Interaction Between PRP11 and SPP91 Yeast Splicing Factors and Characterization of a PRP9-PRP11-SPP91 ComplexScience, 1993
- A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosomeCell, 1992
- Mutational analysis of the yeast U2 snRNA suggests a structural similarity to the catalytic core of group I intronsCell, 1992
- Isolation of a Complementary DNA that Encodes the Mammalian Splicing Factor SC35Science, 1992
- Cloning and domain structure of the mammalian splicing factor U2AFNature, 1992
- Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.Genes & Development, 1989
- A compensatory base change in human U2 snRNA can suppress a branch site mutation.Genes & Development, 1989
- Functional redundancy and structural polymorphism in the large subunit of RNA polymerase IICell, 1987
- Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNACell, 1987