Comparison of K-rasgene mutations in tumour and sputum DNA of patients with lung cancer
- 1 January 2003
- journal article
- research article
- Published by Taylor & Francis Ltd in Biomarkers
- Vol. 8 (2), 156-161
- https://doi.org/10.1080/1354750021000046589
Abstract
Mutations in the K-ras gene are frequently found in lung tumours and are implicated in the development of lung cancer. In order to investigate the clinical usefulness of these mutations in lung cancer, we applied a sensitive method to compare mutations in codon 12 of the K-ras gene in DNA extracted from lung tumours and the matched sputum samples obtained from 22 lung cancer patients. K-ras mutations were identified in the lung tumours of 12 patients (54.5%) and in the sputum samples of 10 patients (45.5%). Nine patients showed an identical mutation in both the tumour and the matched sputum samples. There was a significant association between the presence of a K-ras mutation in a lung tumour and the detection of an identical mutation in the matched sputum sample of the lung cancer patient (κ = 0.64, 95% confidence interval 0.32-0.95, p ras mutations were detected in sputum samples from cancer patients with all lung tumour grades, and both in the presence and the absence of lymph node metastasis. Therefore, K-ras mutations may provide useful diagnostic markers for lung cancer.Keywords
This publication has 11 references indexed in Scilit:
- Detection of Infrequent and Multiple K-rasMutations in Human Tumors and Tumor-Adjacent TissuesAnalytical Biochemistry, 1997
- Cancer statistics, 1996CA: A Cancer Journal for Clinicians, 1996
- High frequency of K‐ras mutations in normal appearing lung tissues and sputum of patients with lung cancerInternational Journal of Cancer, 1995
- Detection of K-ras Oncogene Mutations in Bronchoalveolar Lavage Fluid for Lung Cancer DiagnosisJNCI Journal of the National Cancer Institute, 1995
- Molecular aspects of chemical carcinogenesis: The roles of oncogenes and tumour suppressor genesToxicology, 1995
- Lung cancerCancer, 1995
- K‐ras mutations in human adenocarcinoma of the lung: Association with smoking and occupational exposure to asbestosInternational Journal of Cancer, 1993
- Validation of Intermediate End Points in Cancer ResearchJNCI Journal of the National Cancer Institute, 1990
- Biomarkers as Intermediate End Points in Chemoprevention TrialsJNCI Journal of the National Cancer Institute, 1990
- ras GENESAnnual Review of Biochemistry, 1987