Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration
- 13 December 2010
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (52), 22558-22563
- https://doi.org/10.1073/pnas.1010795108
Abstract
Border cell migration is a stereotyped migration occurring during the development of the Drosophila egg chamber. During this process, a cluster composed of six to eight follicle cells migrates between nurse cells toward the oocyte. Receptor tyrosine kinases (RTKs) are enriched at the leading edge of the follicle cells and establish the directionality of their migration. Endocytosis has been shown to play a role in the maintenance of this polarization; however, the mechanisms involved are largely unknown. In this study, we show that border cell migration requires the function of the small GTPases Rab5 and Rab11 that regulate trafficking through the early and the recycling endosome, respectively. Expression of a dominant negative form of rab11 induces a loss of the polarization of RTK activity, which correlates with a severe migration phenotype. In addition, we demonstrate that the exocyst component Sec15 is distributed in structures that are polarized during the migration process in a Rab11-dependent manner and that the down-regulation of different subunits of the exocyst also affects migration. Together, our data demonstrate a fundamental role for a plasma membrane-endosome trafficking cycle in the maintenance of active RTK at the leading edge of border cells during their migration.Keywords
This publication has 57 references indexed in Scilit:
- Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivoNature, 2010
- Direct detection of guidance receptor activity during border cell migrationProceedings of the National Academy of Sciences of the United States of America, 2010
- Singularity in Polarization: Rewiring Yeast Cells to Make Two BudsCell, 2009
- Pathways and mechanisms of endocytic recyclingNature Reviews Molecular Cell Biology, 2009
- Rab11-FIP3 is a Rab11-binding protein that regulates breast cancer cell motility by modulating the actin cytoskeletonEuropean Journal of Cell Biology, 2009
- Integrin alpha chains exhibit distinct temporal and spatial localization patterns in epithelial cells of the Drosophila ovaryDevelopmental Dynamics, 2008
- Endocytic Trafficking of Rac Is Required for the Spatial Restriction of Signaling in Cell MigrationCell, 2008
- A genome-wide transgenic RNAi library for conditional gene inactivation in DrosophilaNature, 2007
- Endocytosis Optimizes the Dynamic Localization of Membrane Proteins that Regulate Cortical PolarityCell, 2007
- The RAB25 small GTPase determines aggressiveness of ovarian and breast cancersNature Medicine, 2004