Inhibitory effects of resveratrol on MCP-1, IL-6, and IL-8 production in human coronary artery smooth muscle cells
- 10 May 2013
- journal article
- Published by Springer Science and Business Media LLC in Naunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie
- Vol. 386 (9), 835-839
- https://doi.org/10.1007/s00210-013-0877-9
Abstract
Resveratrol, a representative polyphenol compound, is known to have antiatherogenic effects through its various actions including an anti-inflammatory action. The processes of initiation and progression of atherosclerosis are mediated by proinflammatory cytokines. The aim of this study was to determine whether resveratrol affects cytokine production in human coronary artery smooth muscle cells (HCASMCs). Each cytokine concentration in the culture medium of HCASMCs was measured by flow cytometry using the cytometric bead array system, and extracellular signal-regulated kinase (ERK) activity was evaluated by Western blotting. Basal levels of monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-6, and IL-8 were significantly decreased in the presence of resveratrol at 1-50 μM in a concentration-dependent manner and were significantly decreased in the presence of U0126, an ERK inhibitor. Resveratrol significantly decreased both basal and interferon-γ (IFN-γ) (200 ng/ml)-stimulated levels of MCP-1, IL-6, and IL-8 and significantly attenuated both basal and IFN-γ-stimulated activity of ERK. TNF-α, IL-1β, IL-10, and IL-12p70 were detected only as trace levels in the culture medium with or without IFN-γ. Therefore, resveratrol is thought to inhibit production of MCP-1, IL-6, and IL-8 in HCASMCs through attenuating ERK activity. Inhibition of cytokine production in coronary artery smooth muscle cells may in part explain antiatherogenic action of resveratrol.Keywords
This publication has 20 references indexed in Scilit:
- Cardiovascular effects and molecular targets of resveratrolNitric Oxide, 2012
- Atherosclerosis: current pathogenesis and therapeutic optionsNature Medicine, 2011
- Inhibition of proliferation and migration by piceatannol in vascular smooth muscle cellsToxicology in Vitro, 2009
- Resveratrol attenuates angiotensin II-induced interleukin-6 expression and perivascular fibrosisHypertension Research, 2009
- Resveratrol, a Polyphenolic Phytostilbene, Inhibits Endothelial Monocyte Chemotactic Protein-1 Synthesis and SecretionJournal of Vascular Research, 2006
- Resveratrol attenuates TNF-α-induced activation of coronary arterial endothelial cells: role of NF-κB inhibitionAmerican Journal of Physiology-Heart and Circulatory Physiology, 2006
- Resveratrol Inhibits TNF- –Induced Proliferation and Matrix Metalloproteinase Expression in Human Vascular Smooth Muscle CellsJournal of Nutrition, 2005
- Resveratrol inhibits angiotensin II-induced endothelin-1 gene expression and subsequent proliferation in rat aortic smooth muscle cellsEuropean Journal of Pharmacology, 2005
- Effect of Alcohol on Lipids and Lipoproteins in Relation to AtherosclerosisCritical Reviews in Clinical Laboratory Sciences, 2002
- Oxidant Signaling in Vascular Cell Growth, Death, and SurvivalCirculation Research, 2000