Mineralization of nanohydroxyapatite on electrospun poly(l-lactic acid)/gelatin by an alternate soaking process: A biomimetic scaffold for bone regeneration
- 13 June 2012
- journal article
- research article
- Published by SAGE Publications in Journal of Bioactive and Compatible Polymers
- Vol. 27 (4), 356-374
- https://doi.org/10.1177/0883911512447211
Abstract
Biomimetic nanocomposite scaffolds were fabricated by electrospinning poly(l-lactic acid) and a blend of poly(L-lactic acid)/gelatin to eliminate the use of collagen. The scaffolds were mineralized via alternate soaking in calcium and phosphate solutions, whereby 66.8% nanohydroxyapatite formation was successfully induced which is similar to that of native human bone (60%). The poly(L-lactic acid)/gelatin scaffolds had uniform nanohydroxyapatite formation throughout the scaffold. The mineralization enhanced the tensile modulus and tensile strength without increasing the brittleness. The in vitro biocompatibility of scaffolds was evaluated with murine adipose tissue–derived stem cells. The scaffolds with nanohydroxyapatite aided cell attachment and promoted cell–cell interaction. The mineralization and osteocalcin expression of the murine adipose tissue–derived stem cells were maximum in the poly(L-lactic acid)/gelatin/nanohydroxyapatite scaffold. Therefore, the gelatin and nanohydroxyapatite in poly(L-lactic acid)/gelatin/nanohydroxyapatite scaffolds provided cues for the differentiation of murine adipose tissue–derived stem cells. The biochemical nature of poly(L-lactic acid)/gelatin/nanohydroxyapatite scaffold accelerated osteogenic differentiation and could be a potential candidate for bone regeneration.Keywords
This publication has 56 references indexed in Scilit:
- Nanofibrous electrospun barrier membrane promotes osteogenic differentiation of human mesenchymal stem cellsJournal of Bioactive and Compatible Polymers, 2011
- Electrospun poly (lactic-co-glycolic acid)/ multiwalled carbon nanotubes composite scaffolds for guided bone tissue regenerationJournal of Bioactive and Compatible Polymers, 2011
- Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on growth and osteogenic differentiation of human mesenchymal stem cellsJournal of Materials Science: Materials in Medicine, 2011
- Rapid Mineralization of Electrospun Scaffolds for Bone Tissue EngineeringJournal of Biomaterials Science, Polymer Edition, 2011
- The use of silk fibroin/hydroxyapatite composite co-cultured with rabbit bone-marrow stromal cells in the healing of a segmental bone defectThe Journal of Bone and Joint Surgery. British volume, 2010
- Biomimetic Apatite-coated PCL Scaffolds: Effect of Surface Nanotopography on Cellular FunctionsJournal of Bioactive and Compatible Polymers, 2009
- The fabrication of nano-hydroxyapatite on PLGA and PLGA/collagen nanofibrous composite scaffolds and their effects in osteoblastic behavior for bone tissue engineeringBone, 2009
- Generation of Pancreatic Hormone-Expressing Islet-Like Cell Aggregates from Murine Adipose Tissue-Derived Stem CellsThe International Journal of Cell Cloning, 2009
- Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin–hydroxyapatite for tissue engineering scaffoldsBiomaterials, 2005
- Transglutaminase-mediated gelatin matrices incorporating cell adhesion factors as a biomaterial for tissue engineeringJournal of Bioscience and Bioengineering, 2003