Direct determination of molecular haplotypes by chromosome microdissection
Open Access
- 21 March 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 7 (4), 299-301
- https://doi.org/10.1038/nmeth.1443
Abstract
Comparing the haplotypes of a few randomly microdissected chromosomes to a full genome-wide haplotype allows one to determine the long-range haplotype of chromosomes for which only one copy was captured. Direct observation of haplotypes is still technically challenging. Here we report a method for the determination of haplotypes through chromosome microdissection. We determined human long-range chromosomal haplotypes with more than 98.85% accuracy at 24,245 genome-wide heterozygous single-nucleotide polymorphism (SNP) loci.Keywords
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