Correlation of DNA Ploidy to Tumor Histologic Grade, Clinical Variables, and Survival in Dogs with Mast Cell Tumors
- 1 September 1992
- journal article
- Published by SAGE Publications in Veterinary Pathology
- Vol. 29 (5), 386-390
- https://doi.org/10.1177/030098589202900503
Abstract
By using flow cytometry, a retrospective analysis of the DNA content of 40 primary canine mast cell tumors and seven lymph nodes that contained metastatic mast cell tumor from 44 dogs of various breed, sex, and age was performed on formalin-fixed, paraffin-embedded samples of the tumors and nodes. These samples were chosen according to the following criteria: samples contained sufficient well-preserved tumor tissue in the paraffin block for processing, sufficient patient history data were available, clean and homogeneous cell suspensions were obtained after processing, and interpretable DNA histograms were produced on analysis. The ploidy data obtained were compared with the histopathologic grade, the anatomical site of occurrence, the clinical stage of the tumors, and the survival of the dogs. Over 70% (29/40) of the mast cell tumors were diploid. Three metastatic mast cell tumors in lymph nodes had the same ploidy status as their corresponding primary tumors. In five dogs, mast cell tumors from multiple sites in each dog displayed similar ploidy status. Of 26 dogs evaluated for survival times, 69% (18/26) had diploid tumors and 31 % (8/26) had aneuploid tumors. When numbers of diploid versus aneuploid tumors were compared, no significant difference was found between any two grades, clinical stages, or anatomic sites. A significant difference ( P = 0.02) was found, however, between aneuploid and diploid tumors when comparing Stage I and non-Stage I disease. The Kaplan-Meier survival plot indicated a tendency towards an increased survival within the first year in dogs with diploid versus aneuploid tumors ( P = 0.06).Keywords
This publication has 11 references indexed in Scilit:
- DNA flow cytometric study of the hyperplastic and neoplastic canine prostateThe Prostate, 1991
- Cellular DNA content and prognosis of high-grade soft tissue sarcoma: the Scandinavian Sarcoma Group experience.Journal of Clinical Oncology, 1990
- Flow cytometry using paraffin‐embedded tissue: Five years onCytometry, 1989
- Comparison of Histology and Clinical Variables to DNA Ploidy in Canine Mammary TumorsVeterinary Pathology, 1988
- Flow cytometric analysis of head and neck carcinoma DNA index and S‐fraction from paraffin‐embedded sections: Comparison with malignancy gradingCytometry, 1985
- Application of DNA flow cytometry to paraffin‐embedded archival material for the study of aneuploidy and its clinical significanceCytometry, 1985
- Canine Cutaneous Mast Cell Tumor: Morphologic Grading and Survival Time in 83 DogsVeterinary Pathology, 1984
- Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.Journal of Histochemistry & Cytochemistry, 1983
- Clinical application of flow cytometry: A reviewCytometry, 1981
- Cellular DNA content as a marker of neoplasia in manAmerican Journal Of Medicine, 1980