A Critical Role for the TIFY Motif in Repression of Jasmonate Signaling by a Stabilized Splice Variant of the JASMONATE ZIM-Domain Protein JAZ10 inArabidopsis
Open Access
- 1 January 2009
- journal article
- Published by Oxford University Press (OUP) in THE PLANT CELL ONLINE
- Vol. 21 (1), 131-145
- https://doi.org/10.1105/tpc.108.064097
Abstract
JASMONATE ZIM-domain (JAZ) proteins act as repressors of jasmonate (JA) signaling. Perception of bioactive JAs by the F-box protein CORONATINE INSENSITIVE1 (COI1) causes degradation of JAZs via the ubiquitin-proteasome pathway, which in turn activates the expression of genes involved in plant growth, development, and defense. JAZ proteins contain two highly conserved sequence regions: the Jas domain that interacts with COI1 to destabilize the repressor and the ZIM domain of unknown function. Here, we show that the conserved TIFY motif (TIFF/YXG) within the ZIM domain mediates homo- and heteromeric interactions between most Arabidopsis thaliana JAZs. We have also identified an alternatively spliced form (JAZ10.4) of JAZ10 that lacks the Jas domain and, as a consequence, is highly resistant to JA-induced degradation. Strong JA-insensitive phenotypes conferred by overexpression of JAZ10.4 were suppressed by mutations in the TIFY motif that block JAZ10.4–JAZ interactions. We conclude that JAZ10.4 functions to attenuate signal output in the presence of JA and further suggest that the dominant-negative action of this splice variant involves protein–protein interaction through the ZIM/TIFY domain. The ability of JAZ10.4 to interact with MYC2 is consistent with a model in which a JAZ10.4-containing protein complex directly represses the activity of transcription factors that promote expression of JA response genes.Keywords
This publication has 61 references indexed in Scilit:
- Jasmonate signaling: a conserved mechanism of hormone sensingCurrent Opinion in Plant Biology, 2008
- Independently Silencing Two Photosynthetic Proteins inNicotiana attenuataHas Different Effects on Herbivore ResistancePlant Physiology, 2008
- COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatineProceedings of the National Academy of Sciences of the United States of America, 2008
- New Weapons and a Rapid Response against Insect AttackPlant Physiology, 2008
- Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cellsProceedings of the National Academy of Sciences of the United States of America, 2008
- Regulation and Function of Arabidopsis JASMONATE ZIM-Domain Genes in Response to Wounding and HerbivoryPlant Physiology, 2008
- Functional Diversification of Acyl-Coenzyme A Oxidases in Jasmonic Acid Biosynthesis and ActionPlant Physiology, 2006
- PEAPOD regulates lamina size and curvature in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2006
- Remote sensing of future competitors: Impacts on plant defensesProceedings of the National Academy of Sciences of the United States of America, 2006
- The Dilemma of Plants: To Grow or DefendThe Quarterly Review of Biology, 1992