Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients
Open Access
- 6 March 2009
- journal article
- Published by Springer Science and Business Media LLC in Fluids and Barriers of the CNS
- Vol. 6 (1), 1-2
- https://doi.org/10.1186/1743-8454-6-2
Abstract
Toxoplasmic encephalitis (TE) is one of the most common opportunistic infections in immunocompromised patients. In Cuba, despite the highly active antiretroviral therapy, TE is still the most important cause of cerebral mass lesions in patients infected with the human immunodeficiency virus (HIV). The detection of Toxoplasma gondii by PCR may facilitate the diagnosis and follow-up of TE in acquired immunodeficiency syndrome (AIDS) patients by direct identification of parasite DNA in clinical samples. The aim of the present study was to evaluate a rapid PCR method using the B1 gene to detect T. gondii in cerebrospinal fluid (CSF) samples from patients with suspected TE. CSF samples from AIDS and HIV-negative patients were analyzed. Patients were divided into two groups according to the Centre for Disease Control and Prevention (CDC) criteria for AIDS-related TE: AIDS patients with suspected neurotoxoplasmosis and AIDS and HIV-negative patients with other confirmed neurological diseases but no suspicions of TE. Predictive values, diagnostic accuracy, sensitivity and specificity of the PCR B1 method were calculated. The results obtained from 190 patients showed that this assay has a good sensitivity and specificity (83.3% and 95.7%, respectively) for the diagnosis of TE in AIDS patients. PCR using the B1 gene and B22/B23 set of primers is a single, rapid and reliable method that may be valuable for discrimination between toxoplasmosis and other central nervous system (CNS) diseases.Keywords
This publication has 33 references indexed in Scilit:
- Quantitative Real-Time PCR Is Not More Sensitive than “Conventional” PCRJournal of Clinical Microbiology, 2008
- Diagnosis of Cerebral Toxoplasmosis in AIDS Patients in Brazil: Importance of Molecular and Immunological Methods Using Peripheral Blood SamplesJournal of Clinical Microbiology, 2005
- PCR Assay Using Cerebrospinal Fluid for Diagnosis of Cerebral Toxoplasmosis in Brazilian AIDS patientsJournal of Clinical Microbiology, 2004
- Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patientsJournal of Medical Microbiology, 2004
- Optimization and Evaluation of a PCR Assay for Detecting Toxoplasmic Encephalitis in Patients with AIDSJournal of Clinical Microbiology, 2002
- A second European collaborative study on polymerase chain reaction forToxoplasma gondii, involving 15 teamsFEMS Microbiology Letters, 1998
- PCR detection of Toxoplasma gondii DNA in CSF for the differential diagnosis of AIDS-related focal brain lesionsJournal of Medical Microbiology, 1996
- Risk of Toxoplasmic Encephalitis in AIDS Patients: Indications for ProphylaxisScandinavian Journal of Infectious Diseases, 1996
- Value of the Polymerase Chain Reaction for the Detection of Toxoplasma gondii in Cerebrospinal Fluid from Patients with AIDSClinical Infectious Diseases, 1993
- Improved sensitivity of the polymerase chain reaction for detection ofToxoplasma gondii in biological and human clinical specimensEuropean Journal of Clinical Microbiology & Infectious Diseases, 1992