HIV Type 1 Genetic Diversity and Genotypic Drug Susceptibility in the Republic of Moldova
- 1 September 2001
- journal article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 17 (13), 1297-1304
- https://doi.org/10.1089/088922201750461375
Abstract
HIV-1 genetic diversity and, for the first time, genotypic drug susceptibility was investigated for strains circulating in the Republic of Moldova (of the former Soviet Union). Eighty-three samples from adults recently infected by intravenous drug use (IDU) (n = 60), heterosexual contact (n = 8), and from blood donors (n = 15) that tested positive from 1997 to 1998, and originating from different regions of Moldova were serotyped. By group-specific and subtype-specific peptide ELISA, patients were infected by serotype A (n = 65), serotype B (n = 1), or were nontypable (n = 17). Heteroduplex mobility assay (HMA) confirmed 11 subtype A and the one subtype B infection. Analyses of pol and env sequences for six of the IDUs confirmed that they were infected with subtype A strain. These strains clustered tightly with subtype A strains isolated from the former Soviet Union in phylogenetic analysis. No mutations associated with drug resistance were detected. The Republic of Moldova is culturally more closely related to Romania (where subtype F dominates the epidemic), but depends economically on Russia (where subtype A is established among IDUs). Thus, our results suggest that the spread of HIV in this region is driven by drug networks rather than being due to cultural similarities.Keywords
This publication has 21 references indexed in Scilit:
- An AB Recombinant and Its Parental HIV Type 1 Strains in the Area of the Former Soviet Union: Low Requirements for Sequence Identity in RecombinationAIDS Research and Human Retroviruses, 2000
- Antiretroviral Drug Resistance Testing in Adult HIV-1 InfectionJama-Journal Of The American Medical Association, 2000
- An HIV Type 1 Subtype A Outbreak among Injecting Drug Users in LatviaAIDS Research and Human Retroviruses, 1999
- HIV-1 diversity in RomaniaAIDS, 1998
- HIV Type 1 Diversity and the Reliability of the Heteroduplex Mobility AssayAIDS Research and Human Retroviruses, 1998
- A Sudden Epidemic of HIV Type 1 among Injecting Drug Users in the Former Soviet Union: Identification of Subtype A, Subtype B, and NovelgagA/envB RecombinantsAIDS Research and Human Retroviruses, 1998
- Molecular Epidemiology and MT-2 Cell Tropism of Russian HIV Type 1 VariantsAIDS Research and Human Retroviruses, 1996
- Simultaneous introduction of distinct HIV-1 subtypes into different risk groups in Russia, Byelorussia and LithuaniaAIDS, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Molecular epidemiology of HIV-1 in the former Soviet UnionAIDS, 1994