Genetic Profiling of Gleason Grade 4/5 Prostate Cancer: Which is the Best Prostatic Control Tissue?
- 1 December 2003
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Urology
- Vol. 170 (6), 2263-2268
- https://doi.org/10.1097/01.ju.0000096414.25583.0d
Abstract
We examined the variation in gene expression profiles of prostate cancer caused by zone specific genes. Ten normal central zone, 10 transition zone (benign prostatic hyperplasia) and 6 normal peripheral zone tissues from radical retropubic prostatectomies were compared to each other and to 12 peripheral zone Gleason grade 4/5 cancers. Test chips and HuGeneFL6800 (Affymetrix, Inc., Santa Clara, California) chips were used to assay the transcribed genes. Data were obtained with the Microarray Suite Version 4.0.1 (Affymetrix, Inc.) and analyzed statistically. Substantially different gene expression profiles were found depending upon which of the 3 zonal tissues were used as a control. All 3 profiles were compared for efficiency (ability to locate genes) and for robustness (the magnitude of difference between the control and the Gleason grade 4/5 tissue). Microscopically normal appearing peripheral zone tissue at the gene level shows many characteristics of Gleason grade 4/5 cancer. Gene expression profiles of prostate cancer are affected by the zonal location of the control tissue and the cancer.Keywords
This publication has 11 references indexed in Scilit:
- Cancer-related changes in prostate DNA as men age and early identification of metastasis in primary prostate tumorsProceedings of the National Academy of Sciences of the United States of America, 2003
- Decrease and Gain of Gene Expression Are Equally Discriminatory Markers for Prostate Carcinoma: A Gene Expression Analysis on Total and Microdissected Prostate TissueThe American Journal of Pathology, 2002
- MOLECULAR GENETIC PROFILING OF GLEASON GRADE 4/5 PROSTATE CANCERS COMPARED TO BENIGN PROSTATIC HYPERPLASIAJournal of Urology, 2001
- Delineation of prognostic biomarkers in prostate cancerNature, 2001
- Significance analysis of microarrays applied to the ionizing radiation responseProceedings of the National Academy of Sciences of the United States of America, 2001
- AN ANALYSIS OF 148 CONSECUTIVE TRANSITION ZONE CANCERS: CLINICAL AND HISTOLOGICAL CHARACTERISTICSJournal of Urology, 2000
- A high-density probe array sample preparation method using 10- to 100-fold fewer cellsNature Biotechnology, 1999
- Biological Determinants of Cancer Progression in Men With Prostate CancerJAMA, 1999
- Normal Histology of the ProstateThe American Journal of Surgical Pathology, 1988
- Morphometric and Clinical Studies on 68 Consecutive Radical ProstatectomiesJournal of Urology, 1988