Development of protective immunity in a murine model of melioidosis is influenced by the source of Burkholderia pseudomallei antigens
- 12 June 2007
- journal article
- research article
- Published by Wiley in Immunology & Cell Biology
- Vol. 85 (7), 551-557
- https://doi.org/10.1038/sj.icb.7100084
Abstract
Melioidosis is a potentially fatal disease caused by the bacterium, Burkholderia pseudomallei. The current study was carried out to determine the mechanisms involved in the development of protective immunity in a murine model of melioidosis. Following intravenous infection with B. pseudomallei, both C57BL/6 and BALB/c mice demonstrated delayed-type hypersensitivity responses and lymphocyte proliferation towards B. pseudomallei antigens, indicating the generation of B. pseudomallei-specific lymphocytes. Adoptive transfer of these lymphocytes to naïve C57BL/6 mice was demonstrated by a delayed-type hypersensitivity response. Mice were not protected from a subsequent lethal challenge with a highly virulent strain of B. pseudomallei, suggesting that a single intravenous dose of the bacterium is insufficient to induce a protective adaptive immune response. Attempts to induce resistance in susceptible BALB/c mice used repetitive low-dose exposure to live B. pseudomallei. Immune responses and resistance following subcutaneous immunization with live B. pseudomallei were compared with exposure to heat-killed, culture filtrate and sonicated B. pseudomallei antigens. Compared to heat-killed B. pseudomallei, significant protection was generated in BALB/c mice following immunization with live bacteria. Our studies also demonstrate that the type of immune response generated in vivo is influenced by the antigenic preparation of B. pseudomallei used for immunization.Keywords
Funding Information
- National Health & Medical Research Council of Australia (#279404)
This publication has 34 references indexed in Scilit:
- Role of Inducible Nitric Oxide Synthase and NADPH Oxidase in Early Control ofBurkholderia pseudomalleiInfection in MiceInfection and Immunity, 2006
- A Live Experimental Vaccine againstBurkholderia pseudomalleiElicits CD4+T Cell–Mediated Immunity, Priming T Cells Specific for 2 Type III Secretion System ProteinsThe Journal of Infectious Diseases, 2006
- Genome-Wide Expression Analysis of Burkholderia pseudomallei Infection ina Hamster Model of Acute MelioidosisInfection and Immunity, 2006
- Exposure to Burkholderia pseudomallei induces cell-mediated immunity in healthy individualsClinical Microbiology & Infection, 2004
- Analogous Cytokine Responses toBurkholderia pseudomalleiStrains Contrasting in Virulence Correlate with Partial Cross-Protection in Immunized MiceInfection and Immunity, 2002
- Cytokine Gene Expression in Innately Susceptible BALB/c Mice and Relatively Resistant C57BL/6 Mice during Infection with VirulentBurkholderia pseudomalleiInfection and Immunity, 2000
- Parasite dose determines the Th1/Th2 nature of the response toLeishmania major independently of infection route and strain of host or parasiteEuropean Journal of Immunology, 1998
- Macrophage-lymphocyte interactions mediate anti-Burkholderia pseudomalleiactivityFEMS Immunology & Medical Microbiology, 1998
- Establishment of Stable, Cell-Mediated Immunity that Makes "Susceptible" Mice Resistant to Leishmania majorScience, 1992
- Reactivation of latent melioidosisPublished by Oxford University Press (OUP) ,1990