Type X collagen synthesis by chick sternal cartilage and its relationship to endochondral development.
Open Access
- 1 July 1985
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 101 (1), 277-284
- https://doi.org/10.1083/jcb.101.1.277
Abstract
The appearance of localized, paired zones of primary calcification on either side of the midline of the 19-d [day] embryonic chick sternum is heralded by the development of paired, translucent zones 2 d previously. Histological studies demonstrated that the majority of chondrocytes within these translucent zones are hypertrophic, and that the zones are surrounded by a margin of flattened nonhypertrophic cells. The discrete localization of these paired areas of hypertrophic chondrocytes and subsequent endochondral bone development allows for the direct correlation of the histological and biochemical characteristics of the zones sequentially during development and makes it possible to precisely match the synthetic activity to the cellular morphology, thereby eliminating possible minor but critical variations in developmental staging that could otherwise arise. There is a direct spatial and temporal correlation between the degree of cellular maturation and the synthesis of type X collagen, and the sudden and profound initiation of type X collagen synthesis on days 16-17 of development occurs concurrently with the attainment of hypertrophic characteristics by the majority of cells within the translucent zone. Before acquisition of these hypertrophic characteristics, the cells of this precalcification zone synthesize only type II and the minor cartilage collagens. Chondrocytes isolated from these regions in more immature sternae (i.e., 11+ d embryos) were found to synthesize high levels of type X collagen within 4 d of culture within collagen gels even though hypertrophic development and type X collagen synthesis by cells within this region would not normally be apparent in ovo for several more days. There may be a direct correlation between the development of hypertrophic characteristics and the synthesis of type X collagen, and the maturation of chondrocytes in precalcification zones may be regulated by matrix components and/or stimulated by culture within collagen gels.This publication has 26 references indexed in Scilit:
- Synthesis of a low molecular weight collagen by chondrocytes from the presumptive calcification region of the embryonic chick sterna: the influence of culture with collagen gels.The Journal of cell biology, 1984
- Isolation and Characterization of New Collagens from Chick CartilageJBIC Journal of Biological Inorganic Chemistry, 1982
- Characterization of a highly soluble collagenous molecule isolated from chicken hyaline cartilageBiochemistry, 1982
- Electron optical and analytical observations of rat growth plate cartilage prepared by ultracryomicrotomy: The failure to detect a mineral phase in matrix vesicles and the identification of heterodispersed particles as the initial solid phase of calcium phosphate deposited in the extracellular matrixJournal of Ultrastructure Research, 1982
- Minor collagens of chicken hyaline cartilageBiochemistry, 1981
- Identification of two further collagenous fractions from articular cartilageBioscience Reports, 1981
- Isolation and characterisation of an unusual collagen from hyaline cartilage and intervertebral discFEBS Letters, 1981
- Identification of a new disulphide bonded collagen from cartilageFEBS Letters, 1980
- A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide GelsJBIC Journal of Biological Inorganic Chemistry, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970