Rapid and Sensitive Detection of Norovirus Genomes in Oysters by a Two-Step Isothermal Amplification Assay System Combining Nucleic Acid Sequence-Based Amplification and Reverse Transcription-Loop-Mediated Isothermal Amplification Assays
Open Access
- 15 June 2008
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (12), 3912-3914
- https://doi.org/10.1128/aem.00127-08
Abstract
We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.Keywords
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