Analysis of endometrial microbiota by 16S ribosomal RNA gene sequencing among infertile patients: a single‐center pilot study
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Open Access
- 6 May 2018
- journal article
- research article
- Published by Wiley in Reproductive Medicine and Biology
- Vol. 17 (3), 297-306
- https://doi.org/10.1002/rmb2.12105
Abstract
Purpose The present study aimed to analyze the endometrial and vaginal microbiome among a Japanese infertile population by sequencing and the impact of the endometrial and vaginal environment on implantation. Methods In total, 102 infertile (79 in vitro fertilization [IVF] and 23 non‐IVF) patients and seven healthy volunteers were recruited from August to December, 2017. Endometrial fluid and vaginal discharge samples for sequencing were collected by using an intrauterine insemination catheter. The bacterial status of the endometrium and vagina were analyzed. Results The Lactobacillus‐dominated microbiota (>90% Lactobacillus spp.) in the endometrium vs vagina was 38% (30/79) vs 44.3% (44/79) in the IVF patients, 73.9% (17/23) vs 73.9% (17/23) in the non‐IVF patients, and 85.7% (6/7) vs 85.7% (6/7) in the healthy volunteers. The percentage of endometrial Lactobacillus in the healthy volunteers was highly stable within the same menstrual cycle and even in the following cycle. The major taxonomies were Gardnerella, Streptococcus, Atopobium, Bifidobacterium, Sneathia, Prevotella, and Staphylococcus. Fifteen patients achieved pregnancy by a single vitrified‐warmed blastocyst transfer during this study; the median percentage of Lactobacillus in the pregnant women was 96.45 ± 33.61%. Conclusion A considerable percentage of non‐Lactobacillus‐dominated (NLD) microbiota was found in the endometrium of Japanese infertile women. Increasing the endometrial level of the Lactobacilli to >90% might favor the implantation outcome of NLD infertile patients.Keywords
This publication has 25 references indexed in Scilit:
- The endometrial receptivity array for diagnosis and personalized embryo transfer as a treatment for patients with repeated implantation failureFertility and Sterility, 2013
- A Metagenomic Approach to Characterization of the Vaginal Microbiome Signature in PregnancyPLOS ONE, 2012
- Repeated implantation failure: clinical approachFertility and Sterility, 2012
- Temporal Dynamics of the Human Vaginal MicrobiotaScience Translational Medicine, 2012
- An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaeaThe ISME Journal, 2011
- UCHIME improves sensitivity and speed of chimera detectionBioinformatics, 2011
- Vaginal microbiome of reproductive-age womenProceedings of the National Academy of Sciences of the United States of America, 2010
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- Naïve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial TaxonomyApplied and Environmental Microbiology, 2007
- VAGINAL LACTOBACILLI INHIBITING GROWTH OF GARDNERELLA VAGINALIS, MOBILUNCUS AND OTHER BACTERIAL SPECIES CULTURED FROM VAGINAL CONTENT OF WOMEN WITH BACTERIAL VAGINOSISActa Pathologica Microbiologica Scandinavica Series B: Microbiology, 1986