Methods for measuring glutathione concentration and rate of synthesis
- 1 September 2000
- journal article
- review article
- Published by Ovid Technologies (Wolters Kluwer Health) in Current Opinion in Clinical Nutrition and Metabolic Care
- Vol. 3 (5), 385-390
- https://doi.org/10.1097/00075197-200009000-00010
Abstract
Altered γ-glutamylcysteinylglycine homeostasis has been implicated in a wide variety of human diseases. The measurement of the rates of synthesis or loss ofγ-glutamylcysteinylglycine is necessary in order to make meaningful inferences about changes inγ-glutamylcysteinylglycine concentration in these diseased states. In this review, we discuss methods for measuringγ-glutamylcysteinylglycine concentration in biological samples as well as how improvements in the sensitivity of gas chromatography-mass spectrometric analyses have permitted the development of new and convenient stable isotope tracer methods for the in-vivo measurement ofγ-glutamylcysteinylglycine kinetics.Keywords
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